African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12500

Full Length Research Paper

Corn stover-enhanced cellulase production by Aspergillus niger NRRL 567

Muhammad ishfaq Ghori1, Sibtain Ahmed2,3*, Muhammad Aslam Malana1 and Amer Jamil2
1Department of Chemistry, Bhauddin Zakaria University, Multan, Pakistan. 2Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad-38040, Pakistan. 3School of Medicine, University of New Mexico, MSC10-5550, Albuquerque, NM 8713-000, USA.  
Email: [email protected]

  •  Accepted: 18 March 2011
  •  Published: 29 June 2011

Abstract

he production of extracellular cellulases by Aspergilus niger NRRL 567 on corn stover was studied in liquid state fermentation. In this study, three cellulases, exoglucanase (EXG), endoglucanase (EG) and b-glucosidase (BGL) were produced by A. niger NRRL 567. The optimal pH, temperature and incubation time for cellulases production was found to be 3.5, 30°C and 96 h, respectively. Maximal cellulases activities were achieved with 4% corn stover, 0.1% molasses and 1% yeast sludge. To our knowledge, this is the first report on production of cellulases by using corn stover as a substrate from A. niger NRRL 567.

 

Key words: Corn stover, yeast sludge, cellulases, Aspergillus niger.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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