African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12331

Full Length Research Paper

Brewer’s residues and cocoa pod shells as a substrate for cultivation of Pleurotus ostreatus CCIBt 2339 and enzymes production

Carolina Fernandes Pereira
  • Carolina Fernandes Pereira
  • Universidade Estadual de Santa Cruz (UESC), Rodovia Jorge Amado, km 16, Salobrinho, CEP: 45.662-900, Ilhéus, Bahia, Brazil.
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Antônio Zózimo de Matos Costa
  • Antônio Zózimo de Matos Costa
  • Comissão Executiva do Plano da Lavoura Cacaueira (CEPLAC), Centro de Pesquisas do Cacau, Seção de Fitopatologia. Rodovia Jorge Amado, km 22, CEP: 45.600-000, Itabuna, Bahia, Brazil.
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Givaldo Rocha Niella
  • Givaldo Rocha Niella
  • Comissão Executiva do Plano da Lavoura Cacaueira (CEPLAC), Centro de Pesquisas do Cacau, Seção de Fitopatologia. Rodovia Jorge Amado, km 22, CEP: 45.600-000, Itabuna, Bahia, Brazil.
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José Luiz Bezerra
  • José Luiz Bezerra
  • Universidade Federal do Recôncavo da Bahia (UFRB), Centro de Ciências Agrárias, Ambientais e Biológicas. Campus de Cruz das Almas, CEP: 44.380-000, Cruz das Almas, Bahia, Brazil.
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Ana Paula Trovatti Uetanabar
  • Ana Paula Trovatti Uetanabar
  • Universidade Estadual de Santa Cruz (UESC), Rodovia Jorge Amado, km 16, Salobrinho, CEP: 45.662-900, Ilhéus, Bahia, Brazil.
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Elizama Aguiar-Oliveira
  • Elizama Aguiar-Oliveira
  • Universidade Estadual de Santa Cruz (UESC), Rodovia Jorge Amado, km 16, Salobrinho, CEP: 45.662-900, Ilhéus, Bahia, Brazil.
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  •  Received: 12 September 2020
  •  Accepted: 06 October 2020
  •  Published: 31 March 2021

Abstract

This work investigated the best composition of a substrate for cultivation of Pleurotus ostreatus CCIBt 2339 using brewer's spent grain (SG) and cocoa pod shells (CP) complemented with hot trub (HT) and/or residual brewer’s yeast (RY). The residue HT was detrimental for cultivation and a substrate composition (%, w/w) of 58% SG + 40% CP + 2% RY – with a C/N ratio of 25.77 g/g – resulted in the best values of biological efficiency (BE = 1.204.0 g/kg) and productivity [Pd = 32.5 g/(kg.day)]. The crude multi-enzymatic extract, obtained as a result of the mycelial growth in this substrate was a good source for: laccases (7.644.4 U/g), xylanases (110.9 U/g) and amylases (277.4 U/g). The obtained results demonstrate the biotechnological potential for the proposed substrate for edible mushrooms production as much as for the obtaining of enzymes with industrial application.

 

Key words: Carbon/Nitrogen ratio, cellulases, pectinase, simplex-centroid design, tannase.