This work investigated the best composition of a substrate for cultivation of Pleurotus ostreatus CCIBt 2339 using brewer's spent grain (SG) and cocoa pod shells (CP) complemented with hot trub (HT) and/or residual brewer’s yeast (RY). The residue HT was detrimental for cultivation and a substrate composition (%, w/w) of 58% SG + 40% CP + 2% RY – with a C/N ratio of 25.77 g/g – resulted in the best values of biological efficiency (BE = 1.204.0 g/kg) and productivity [Pd = 32.5 g/(kg.day)]. The crude multi-enzymatic extract, obtained as a result of the mycelial growth in this substrate was a good source for: laccases (7.644.4 U/g), xylanases (110.9 U/g) and amylases (277.4 U/g). The obtained results demonstrate the biotechnological potential for the proposed substrate for edible mushrooms production as much as for the obtaining of enzymes with industrial application.
Key words: Carbon/Nitrogen ratio, cellulases, pectinase, simplex-centroid design, tannase.
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