African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12191

Full Length Research Paper

Immunodiagnostic potential of a 27 kDa protein of Fusarium xylarioides, the cause of coffee wilt disease in Robusta coffee in Uganda

Olal, S.*
  • Olal, S.*
  • National Agricultural Research Organization, National Coffee Resources Research Institute, P.O.BOX 185 Mukono, Uganda.
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Atuhaire, K. D.*
  • Atuhaire, K. D.*
  • National Agricultural Research Organization, National Livestock Resources Research Institute, P.O.BOX 96, Tororo, Uganda; College of Veterinary medicine, Animal resources and Biosecurity, Makerere University, Kampala, Uganda.
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Ochwo, S.
  • Ochwo, S.
  • College of Veterinary medicine, Animal resources and Biosecurity, Makerere University, Kampala, Uganda.
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Kiiza, L.
  • Kiiza, L.
  • National Agricultural Research Organization, National Coffee Resources Research Institute, P.O.BOX 185 Mukono, Uganda.
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Kangire, A.
  • Kangire, A.
  • National Agricultural Research Organization, National Coffee Resources Research Institute, P.O.BOX 185 Mukono, Uganda.
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Musoli, P.
  • Musoli, P.
  • National Agricultural Research Organization, National Coffee Resources Research Institute, P.O.BOX 185 Mukono, Uganda.
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Olaho-Mukani, W.
  • Olaho-Mukani, W.
  • African Union-Interafrican Bureau of Animal Resources, P.O. BOX 30786, Nairobi, Kenya.
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Lubega, G. W.
  • Lubega, G. W.
  • College of Veterinary medicine, Animal resources and Biosecurity, Makerere University, Kampala, Uganda.
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Hakiza, G. J.
  • Hakiza, G. J.
  • National Agricultural Research Organization, National Coffee Resources Research Institute, P.O.BOX 185 Mukono, Uganda.
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  •  Received: 07 November 2013
  •  Accepted: 20 June 2014
  •  Published: 16 July 2014

Abstract

Several Fusarium species infect Robusta coffee; these Fusarium xylarioides Steyaert (Gibberella xylarioides Heim and Saccas) are the most virulent and responsible for the destructive Robusta coffee wilt disease in Uganda. To date, F. xylarioides has not been isolated directly from soil, though the pathogen can persist in soil for a short time. In this study, a promising diagnostic target which can be developed into a serological test for F. xylarioides in coffee plants and soil has been identified and validated for identification. Water-soluble extracts of mycelia from six Fusaruim species were resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The different protein profiles from the other five Fusarium species were compared and contrasted with that of F. xylarioides. Protein bands that appeared peculiar to F. xylarioides were cut and injected into rabbits to produce polyclonal antibodies. Dot blot and Western blot analyses showed one immunodominant antigen (27 kDa) common to all F. xylarioides isolates analyzed. No cross-reactivity of anti-27 kDa antibodies were observed in the entire test Fusarium species. The results suggest that polyclonal antibodies raised against the endoantigens from F. xylarioides of 27 kDa, is a promising tool for the rapid, sensitive, and accurate detection of pathogen in soil and plant parts.

 

Key words: Gibberella xylarioides, coffee wilt disease, antigen, antibodies, Uganda.