African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12400

Full Length Research Paper

Antibacterial activity of Waltheria indica Linn (Sterculiaceae), collected from Blouberg area, Limpopo Province, South Africa

Mongalo, N. I.
  • Mongalo, N. I.
  • University of Zululand, Department of Botany, Private Bag X1001, Internal Box 223, KwaDlangezwa, 3886, South Africa.
  • Google Scholar

  •  Received: 20 March 2014
  •  Accepted: 03 July 2014
  •  Published: 30 July 2014


Mature roots of Waltheria indica were collected from Blouberg area, Limpopo Province, republic of South Africa. Water, ethanol and methanol extracts were investigated for antibacterial activity at 5 mg/ml against 10 human pathogenic strains using disc diffusion method. Ethanol extract exhibited potent antibacterial activity against selected strains while methanol extract exhibited largest zone of inhibition of 15.2±2.18 mm against Bacillus pumilus. Extracts were further investigated for antibacterial activity using minimum inhibitory concentration (MIC) assay. Lowest MIC of 0.65 mg/ml was exhibited by methanol extract against Acinetobacter calcaoceuticals anitratus. Water extract exhibited good MIC of 2.08 mg/ml against Escherichia coli while ethanol extract showed lowest MIC of 1.04 mg/ml against Enterobacter cloacae and 6.25 mg/ml against Klebsiella spp., Serratia marscens and Staphylococcus epidirmidis. Moreover, ethanol extract possessed a total activity of 433 mL/g against Enterococcus faecalis, meaning that the extract can be diluted to 433 mL and would still inhibit growth of these bacteria. These results in a way validate the use of W. indica L in the treatment of variety of infections, especially urinary tract infecting bacterial strains.


Key words: Waltheria indica L, minimum inhibitory concentration (MIC), disc diffusion, total activity, antibacterial.