African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

A new immunoregulatory agent from Rosa damascena reduces CD3 expression in human peripheral blood mononuclear cells (PBMCs)

Albogami Sarah
  • Albogami Sarah
  • Biotechnology Department, Faculty of Science, Taif University, 888-Taif, Kingdom of Saudi Arabia.
  • Google Scholar

  •  Received: 17 February 2017
  •  Accepted: 14 June 2017
  •  Published: 28 June 2017


T-Lymphocytes (T-cells) play a dominant role in the initiation and maintenance of autoimmune inflammatory processes. They become activated by the presentation of antigen such as auto antigen. A main feature of T-cell activation is the production of cytokines. Though, uncontrolled T-cells activation through T-cell receptor (TCR) has been linked to numerous autoinflammatory and autoimmune pathologies. This study investigates the impact of methanol extracts from Rosa damascena on suppress T-cells activation. Peripheral blood mononuclear cells (PBMC) were treated by 10, 50,100 μg/ml of R. damascene methanolic extracts after activation of T cells with anti- CD3 and anti-CD28. The inhibitory role of methanol extracts of R. damascena to suppress the activated T-cells was measured using flow cytometric at three different time points (24, 48, and 72 h). The result showed that the methanol extraction from R. damascena petal parts can inhibit the activated T-cells in a dose dependent fashion as we observe reduced levels of cluster of differentiation of CD 3. It is concluded that, extracts of R. damascena could be used as a new agent (immunoregulatory) to suppress T-cell activity and treating variety of immune disorders with a low risk of side effects in future.

Key words: T-lymphocytes (T-cells), autoimmune inflammatory, Rosa damascena, (CD) 3.