African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12504

Full Length Research Paper

Optimization of cholesterol oxidase production by Brevibacterium sp. employing response surface methodology

  Shengli Yang1* and Hui Zhang2  
  1The College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310014, People’s Republic of China. 2Quality and Technical Supervision and Inspection Institute of Zhejiang Province, Physical and Chemical Test Center, Hangzhou 310013, People’s Republic of China.
Email: [email protected]

  •  Accepted: 30 March 2012
  •  Published: 24 April 2012

Abstract

 

An ultrasound-assisted emulsification as a pretreatment for cholesterol oxidaseproduction by submerge fermentation using Brevibacterium sp. in a batch system was studied. Medium improvement for the production employing response surface methodology (RSM) was optimized in this paper. The concentration of Tween-80, cholesterol and the time of ultrasonic pretreatment medium were further optimized by RSM. Results from RSM showed that the initial concentration of cholesterol, Tween-80 and pretreatment time exerted a significant effect on cholesterol oxidase production, but these factors did not exert a significant effect on cell growth. The improved medium consisted of 4.076 g/L cholesterol, 0.2932‰ (v/v) Tween-80, 22.361 (min) treatment time, and cholesterol oxidase production reached 1.483 U/ml after 36 h culture, which was 83.57% greater than the control medium.

 

Key words: Ultrasonic, cholesterol oxidase, response surface methodology.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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