African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12227

Full Length Research Paper

Genetic diversity of Przewalski’s gazelle using noninvasive DNA and its implications for conservation

Hairui Duo
  • Hairui Duo
  • Institute of Forestry Ecology, Environment and Protection and the Key Laboratory of Forest Ecology and Environment of State Forestry Administration, Chinese Academy of Forestry, Beijing 100091, China.
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Li Na
  • Li Na
  • Institute of Forestry Ecology, Environment and Protection and the Key Laboratory of Forest Ecology and Environment of State Forestry Administration, Chinese Academy of Forestry, Beijing 100091, China.
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Yanyun Hong
  • Yanyun Hong
  • Institute of Forestry Ecology, Environment and Protection and the Key Laboratory of Forest Ecology and Environment of State Forestry Administration, Chinese Academy of Forestry, Beijing 100091, China.
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Diqiang Li
  • Diqiang Li
  • Institute of Forestry Ecology, Environment and Protection and the Key Laboratory of Forest Ecology and Environment of State Forestry Administration, Chinese Academy of Forestry, Beijing 100091, China.
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  •  Received: 04 May 2012
  •  Accepted: 08 March 2015
  •  Published: 01 April 2015

Abstract

The Przewalski’s gazelle (Procapra przewalskii) is endemic to China and the total current population of the species is only about several hundred. In order to understand the genetic structure and diversity of the Przewalski’s gazelle in China for the purpose of guiding conservation initiatives, we examined the genetic variability and differentiation based on microsatellite loci, using noninvasive genetic sampling. 188 Przewalski’s gazelle scats from six populations around Qinghai Lake (Tianjun, Haergai, Bird Island, Hudong-Ketu, Sand Island and Yuanzhe) were collected, and 128 individuals were identified and 94 alleles were detected at the 12 loci surveyed. The results showed that the expected heterozygosity and polymorphism information content is over 0.79 and 0.75, respectively. The AMOVA results showed that 5% of all observed diversity was due to difference between the populations and 95% was caused by genetic variance within population. According to the genetic similarity and geographical closeness, we suggested that six populations should be managed as three separate conservation units and habitat corridors should be built to link the Yuanzhe, Hudong-Ketu, Haergai and Sand Island populations. 
 
Key words: Habitat fragment, genetic diversity, noninvasive genetics, Przewalski’s gazelle, Procapra przewalskii.