African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12300

Full Length Research Paper

Evaluation of cytotoxic and mutagenic effects of two artificial sweeteners by using eukaryotic test systems

Victor Alves de Oliveira
  • Victor Alves de Oliveira
  • Department of Nutrition, Campus Senador Helvídio Nunes de Barros - CSHNB, Federal University of Piauí -UFPI, Picos (PI), Brazil.
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Virleny Maria alves de Oliveira
  • Virleny Maria alves de Oliveira
  • Department of Nutrition, Campus Senador Helvídio Nunes de Barros - CSHNB, Federal University of Piauí -UFPI, Picos (PI), Brazil.
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Thayse Wilma Nogueira de Oliveira
  • Thayse Wilma Nogueira de Oliveira
  • Department of Nutrition, Campus Senador Helvídio Nunes de Barros - CSHNB, Federal University of Piauí -UFPI, Picos (PI), Brazil.
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Andressa Nathanna Castro Damasceno
  • Andressa Nathanna Castro Damasceno
  • Department of Nutrition, Campus Senador Helvídio Nunes de Barros - CSHNB, Federal University of Piauí -UFPI, Picos (PI), Brazil.
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Charles Emanuel de Oliveira Silva
  • Charles Emanuel de Oliveira Silva
  • Department of Nutrition, Campus Senador Helvídio Nunes de Barros - CSHNB, Federal University of Piauí -UFPI, Picos (PI), Brazil.
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Stella Regina Arcanjo Medeiros
  • Stella Regina Arcanjo Medeiros
  • Department of Nutrition, Campus Senador Helvídio Nunes de Barros - CSHNB, Federal University of Piauí -UFPI, Picos (PI), Brazil.
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Bruno Moreira Soares
  • Bruno Moreira Soares
  • Human Cytogenetics Laboratory and Oncology Research Center, Federal University of Pará, Belém-Pará, Brazil.
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Felipe Cavalcanti Carneiro da Silva
  • Felipe Cavalcanti Carneiro da Silva
  • Postgraduate Program in Pharmaceutical Science, Federal University of Piauí, Teresina (PI)- 64.049-550, Brazil.
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Raí Pablo Sousa de Aguiar
  • Raí Pablo Sousa de Aguiar
  • Centro Universitário de Saúde, Ciências Humanas e Tecnológicas do Piauí –UNINOVAFAPI, ua Vitorino Orthiges Fernandes, 6123 - Uruguai, Teresina - PI, 64073-505, Brazil.
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Muhammad Torequl Islam*
  • Muhammad Torequl Islam*
  • Postgraduate Program in Biotechnology, Northeast Biotechnology Network (RENORBIO), Federal University of Piauí, Teresina (Piauí)-64009-550, Brazil.
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Ana Amélia de Carvalho Melo-Cavalcante
  • Ana Amélia de Carvalho Melo-Cavalcante
  • Postgraduate Program in Pharmaceutical Science, Federal University of Piauí, Teresina (PI)- 64.049-550, Brazil.
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Ana Paula Peron
  • Ana Paula Peron
  • Laboratory of Cytogenetics and Mutagenesis. Department of Biological Sciences and Postgraduate Program in Genetics and Plant Breeding. Federal University of Piauí, Teresina (Piauí)-64009-550, Brazil.
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Joao Marcelo de Castro e Sousa
  • Joao Marcelo de Castro e Sousa
  • Postgraduate Program in Pharmaceutical Science, Federal University of Piauí, Teresina (PI)- 64.049-550, Brazil.
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  •  Received: 23 September 2016
  •  Accepted: 10 February 2017
  •  Published: 15 March 2017

Abstract

Considering the vast use of sweeteners by the Brazilian population and the constant need for toxicological studies of food additives, this study aimed to evaluate the cytotoxic and mutagenic potentials of the sodium saccharin and/or sodium cyclamate sweeteners in plant (Allium cepa) and animal (Mus musculus) test systems based on concentrations permitted by the Brazilian laws. In A. cepa, both the sweeteners individually and their combinations concentration and exposure time (ET: 48, 72 and 168 h) dependently exerted cytotoxic and mutagenic effects. Similarly, an increased in micronuclei formation was also observed by the sweeteners in peripheral blood cells in mice. More toxic effects were observed with the combination doses at 168 ET. There may be a synergistic effect and DNA damage with an increasing concentration and ET. In conclusion, the concentrations considered safe by the Brazilian laws had significant cytotoxic and mutagenic activity on the eukaryotic cells.

Key words: Allium cepa, Mus musculus, sweeteners, toxicity.