African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12158

Full Length Research Paper

Microbial diversity in leaves, trunk and rhizosphere of coconut palms (Cocos nucifera L.) associated with the coconut lethal yellowing phytoplasma in Grand-Lahou, Côte d’Ivoire

Nadia P. Morales-Lizcano
  • Nadia P. Morales-Lizcano
  • Department of Cell and Systems Biology, University of Toronto, 25 Willcocks Street Toronto, ON M5S 3B2, Canada.
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Ahmed Hasan
  • Ahmed Hasan
  • Department of Ecology and Evolutionary Biology, University of Toronto, Mississauga, 3359 Mississauga Road, ON, L5L 1C6, Canada.
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Henry S. To
  • Henry S. To
  • Sporometrics, 219 Dufferin Street, Suite 20C, Toronto, ON M6K 3J1, Canada.
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Tacra T. Lekadou
  • Tacra T. Lekadou
  • ‘Marc Delorme’ Station, National Centre of Agronomic Research, 07 BP 13 Port Bouet, Abidjan, Côte d’Ivoire.
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Julia Copeland
  • Julia Copeland
  • Centre for the Analysis of Genome Evolution and Function (CAGEF), University of Toronto, 25 Willcocks Street Toronto, ON M5S 3B, Canada.
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Pauline Wang
  • Pauline Wang
  • Centre for the Analysis of Genome Evolution and Function (CAGEF), University of Toronto, 25 Willcocks Street Toronto, ON M5S 3B, Canada.
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Hortense A. Diallo
  • Hortense A. Diallo
  • University of Nangui Abrogoua, 02 BP 801, Abidjan, Côte d’Ivoire.
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Jean L. Konan Konan
  • Jean L. Konan Konan
  • ‘Marc Delorme’ Station, National Centre of Agronomic Research, 07 BP 13 Port Bouet, Abidjan, Côte d’Ivoire.
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Keiko Yoshioka
  • Keiko Yoshioka
  • Department of Cell and Systems Biology, University of Toronto, 25 Willcocks Street Toronto, ON M5S 3B2, Canada.
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Wolfgang Moeder
  • Wolfgang Moeder
  • Department of Cell and Systems Biology, University of Toronto, 25 Willcocks Street Toronto, ON M5S 3B2, Canada.
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James Scott
  • James Scott
  • Dalla Lana School of Public Health, University of Toronto, 223 College Street, Toronto ON M5T 1R4, Canada.
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Yaima Arocha Rosete
  • Yaima Arocha Rosete
  • Sporometrics, 219 Dufferin Street, Suite 20C, Toronto, ON M6K 3J1, Canada.
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  •  Received: 27 March 2017
  •  Accepted: 15 June 2017
  •  Published: 05 July 2017

Abstract

The diversity of culturable bacterial and fungal communities was assessed from leaves, trunk and rhizosphere of coconut palms infected and non-infected by the Côte d’Ivoire lethal yellowing (CILY) phytoplasma. Bacterial and fungal microbes were isolated from leaves, trunk and rhizosphere samples collected from two villages of Grand-Lahou, Braffedon and Yaokro, by using a high-throughput 'dilution-to-extinction' cultivation method coupled with PCR and sequencing with primers that amplified both the 16S ribosomal RNA and intergenic transcribed spacer (ITS) genes. The relative abundance was higher for Bacillus and Candida in Braffedon, and Burkholderia and Neodeightonia in Yaokro. Commonly genera identified from rhizosphere included Bacillus, Burkholderia, Pseudomonas, Streptomyces, Cryptococcus, Penicillium, Purpureocillium and Trichoderma. The most abundant endophytes identified were Pantoea, Candida, Cryptococcus, Bacillus, Pseudomonas, Penicillium, Aspergillus and Rhodotorula. Genera limited to symptomless palms included Arthrininum, Chaetomium, Phialemonium, Fusarium, Klebsiella and Candida. Results indicate that the CILY phytoplasma may be a factor determining the level of diversity of a microbial community in a given location. Our research provides the basis to investigate the possible effect of endophytic and rhizosphere microbes against the CILY phytoplasma to further effectively improve the management of CILY in Grand-Lahou.

 

Key words: Côte d’Ivoire lethal yellowing phytoplasma, endophytes, biocontrol, coconut.