African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12405

Full Length Research Paper

Semi-quantitative analysis of endometrial receptivity marker mRNA expression in the mid-secretory endometrium of patients with uterine fibromas

  Naser Shokrzadeh1 and Zohreh Alizadeh2,3*      
  1Department of Basic Sciences, Gonabad University of Medical Sciences, Gonabad, Khorasan Razavi, Iran. 2Research Center for Molecular Medicine, Hamadan University of Medical Sciences,Shahid Fahmideh Ave., Hamadan, P.O.Box. 65178-518, Iran. 3Department of Anatomical Sciences, Hamadan University of Medical Sciences, Hamadan, Iran.
Email: [email protected]

  •  Accepted: 01 March 2012
  •  Published: 20 March 2012

Abstract

 

In fertile women, expression of molecular marker of endometrial receptivity, HOXA11, leukemia inhibitory factor (LIF) and basic transcriptional element binding protein 1 (BTEB1), rises during the luteal phase with the peak occurring during the implantation window. We evaluated the transcript levels of HOXA-11, LIF and BTEB1 in the mid-secretory endometrium of infertile patients with uterine fibroid infertility (n = 8) and from normal fertile women (n = 8). Expression levels of HOXA11, LIF and BTEB1 mRNA were measured in endometrium during the mid-secretory phase using semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). Endometrial HOXA11, LIF and BTEB1 mRNA expression levels (normalized to ß-actin expression) were significantly decreased in endometrium of infertile patients with uterine fibroid as compared with healthy fertile controls at the time of implantation (P<0.05). The results suggest that the alteration in expression pattern of some genes could account for some aspects of infertility in patients with uterine fibroma.

 

Key words: Myoma, fibromas, implantation, HOXA11, leukemia inhibitory factor, basic transcriptional element binding protein 1.

Abbreviation

 LIF, Leukemia inhibitory factor; BTEB1, basic transcriptional element binding protein 1.