African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12275

Full Length Research Paper

Genetic diversity in Nepalese population of Swertia chirayita (Roxb. Ex Fleming) H. Karst based on inter-simple sequence repeats (ISSR) markers

Surendra Neupane
  • Surendra Neupane
  • Molecular Biotechnology Unit, Nepal Academy of Science and Technology (NAST), Khumaltar, Lalitpur, Nepal
  • Google Scholar
Jaishree Sijapati
  • Jaishree Sijapati
  • Molecular Biotechnology Unit, Nepal Academy of Science and Technology (NAST), Khumaltar, Lalitpur, Nepal
  • Google Scholar
Tribikram Bhattarai
  • Tribikram Bhattarai
  • Central Department of Biotechnology, Tribhuvan University, Kirtipur, Kathmandu, Nepal
  • Google Scholar
Sangita Shrestha
  • Sangita Shrestha
  • Molecular Biotechnology Unit, Nepal Academy of Science and Technology (NAST), Khumaltar, Lalitpur, Nepal
  • Google Scholar


  •  Received: 28 December 2016
  •  Accepted: 20 March 2017
  •  Published: 19 April 2017

Abstract

Swertia chirayita is a highly valued but vulnerable medicinal plant species of Nepal. Its populations are declining in natural habitats due to over exploitation. Twenty-seven inter-simple sequence repeats (ISSR) primers were used to assess the genetic diversity and population genetic structure of 42 genotypes representing six natural populations of S. chirayita, from Nepal. Of the total 479 bands amplified by 27 ISSR primers, 473 (98.18%) were polymorphic, indicating very high level of genetic diversity at species level. Percentage polymorphism value for different primers ranged from 83.3 to 100% with an average of 98.18%. Polymorphism information content (PIC) value ranged from 0.88 to 0.93 with an average of 0.91. Cluster analysis performed with NTSYS pc statistical package using Jaccard’s similarity coefficients generated from ISSR binary data matrix showed that, all 27 ISSR primers separated 42 individuals into two major clusters and six sub clusters at the similarity level of 0.24. The average value of Nei’s genetic diversity (H) and Shannon’s information index (I) equaled 0.276 and 0.423, respectively at species level. The coefficient of genetic differentiation (GST) amongst populations of S. chirayita was found to be high (0.548) with restricted gene flow (Nm=0.4829). Analysis of molecular variance showed that genetic diversity within populations is slightly higher (50.9%) than among populations (47.6%). The present genetic diversity assessment of S. chirayita populations has been of immense importance to understand the cause of its vulnerability and has furnished valuable insights for its conservation and sustainable utilization.

 

Key words: Polymerase chain reaction, inter-simple sequence repeats (ISSR), genetic diversity, polymorphism, population genetics.