African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12236

Full Length Research Paper

Isolation, identification and screening of potential cellulase-free xylanase producing fungi and its production

Rajashri D. Kamble1* and Anandrao R. Jadhav2
1Department of Biotechnology, Tatyasaheb Kore Institute of Engineering and Technology, Warananagar,Pin- 416113.Tal. Panhala, Dist. Kolhapur, Maharashtra, India. 2Department of Microbiology, Smt. K.R.P. Kanya Mahavidyalaya, Islampur, Pin 415 414, Tal: Walwa Dist: Sangli, Maharashtra, India.  
Email: [email protected]

  •  Accepted: 05 September 2012
  •  Published: 25 September 2012

Abstract

In order to isolate cellulase-free xylanase producing fungi, screening and isolation was done using composting soil as microbial source. Eight fungal species were selected for further study based on clearing zones formation on agar media containing covalently linked xylan with dye cibacron brilliant red-3BA. Both solid state and submerged fermentations were done with eight fungal isolates to identify strain that could produce highest amount of cellulase-free xylanase at a pH of 5.5 and at 28 ± 2°C temperature. Under solid state conditions, 50% of the strains produced xylanase (45.78 to 923 U gds-1) and lower amount of cellulase (20 to 33 U gds-1). The amount of soluble protein was also determined which ranged between 67 to 99 mg gds-1. In submerged conditions, 25% strains produced xylanase (45 to 205 U mL-1) and negligible amount of cellulase (12 to 13 U mL-1). The amount of soluble protein was determined which ranged between 45 and 85 U ml-1 in submerged conditions. Growth was determined in terms of mycelial dry weight which ranged between 0.70 and 2.90 mg mL-1.

 

Key words: Cellulase-free xylanase, xylan, solid state fermentation, submerged fermentation.

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