Full Length Research Paper
Abstract
To verify the molecular markers linked to the genic multiple-allele male-sterile geneMs, an F1 plant, which was generated by crossing the inbred line a20 and the male-sterile plant of the genic multiple-allele male-sterile AB line, was backcrossed with an a20 plant to develop BC4 and BC5 populations. Sequence-characterized amplified region (SCAR) marker syau_scr01 and simple sequence repeat (SSR) marker syau_m13, which were linked to Ms, exhibited polymorphism between the 2 parents. The accuracies of these 2 markers in determining the plant genotype was 85 and 91.7%, respectively. The accuracy reached 100% when the 2 markers were used in combination. These results indicate that these 2 markers can be applied in the marker-assisted selection of the genic multiple-allele male-sterile line of Chinese cabbage.
Key words: Chinese cabbage, genic multiple-allele male sterility, marker-assisted selection, simple sequence repeat, sequence-characterized amplified region.
Abbreviation
SCAR, Sequence-characterized amplified region; SSR, simple sequence repeat; MAS, marker-assisted selection; BSA, bulk segregant analysis.
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