African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12227

Short Communication

Analysis of genetic variation in different banana (Musa species) variety using random amplified polymorphic DNAs (RAPDs)

Pankaj Kumar Jain1, Mohan Lal Saini2*, Hardik Pathak1 and Virendra Kumar Gupta1
  1Departmant of Biotechnology, Mahatma Gandhi Institute of Applied Sciences, Jaipur, India. 2Biotechnology Lab, Department of Botany, UOR, Jaipur, India.

  •  Accepted: 27 July 2007
  •  Published: 05 September 2007

Abstract

 

The banana (Musa acuminata Colla) is considered as an important crop plant due to its high economic value as good dietary source. Here, we analyze the genetic relationship of four different banana varieties that are cultivated in south India. Random amplified polymorphic DNAs (RAPDs) fingerprinting of these banana varieties (Grand Naine, Red Banana, Nendran and Rasthali) carried out by three primers (OPA-19, OPB-18, OPD-16) led to DNA amplification. 43.47% of the amplification products were monomorphic (common to all the genotypes), whereas 30.43% were unique, but only 26.08% revealed the relationship between these genotypes.

 

Key words: DNA fingerprinting, RAPD analysis, Taq DNA polymerase, primers.