African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12296

Full Length Research Paper

Screening of oleaginous yeast with xylose assimilating capacity for lipid and bio-ethanol production

  Ming-xiong He1,2*, Qichun Hu1,2, Xinhua Gou3, Xinying Liu3, Qing Li1,Ke Pan1, Qili Zhu1 and Jin Wu1  
1Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Chinese Academy of Agriculture Sciences, Section 4 - 13, Renming Nanlu, Chengdu 610041, China. 2Key Laboratory of Energy Microbiology and Application, Ministry of Agriculture, Chengdu 610041, China. 3College of Biological Industry, Chengdu University, Chengdu 610106, China.  
Email: [email protected]

  •  Accepted: 23 September 2010
  •  Published: 06 December 2010

Abstract

 

Microbial oil is a promising new biodiesel resource, which have great potential in industrial-scale production. In our preliminary study, 57 oleaginous yeast with xylose assimilating capacity were isolated from 13 soil samples, 16 strains were identified as potential lipid biomass producer. Four strains which showed higher lipid content were used for further ethanol fermentation at different conditions. Strain 9-44 belonging to Pichia guillermondii showed the highest ethanol production (21.91 g/l), and the theoretical ethanol yield was 85.90%. Our study will be of great significance for coupling of lipid and bio-ethanol production, and also provide a choice of cellulocis biomass utilization.

 

Key words: Microbial oil, oleaginous yeast, Pichia guillermondii, bio-ethanol, cellulocis biomass.

Abbreviation

 

Abbreviations: GHG, Greenhouse gas; YPG, yeast peptone glucose; YPX, yeast peptone xylose; FID, flame ionization detector; DNS, dinitrosalicylic acid; PCR,polymerase chain reaction; YPD, yeast peptone dextrose.