African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12191

Short Communication

Genetic diversity in cowpea [Vigna unguiculata (L.) Walp.] varieties determined by ARA and RAPD techniques

Laïty Fall1, Diaga Diouf1*, Mame Arama Fall-Ndiaye 1, François Abaye Badiane1 and Mamadou Gueye2
  1Laboratoire de Biotechnologies Végétales, Département de Biologie Végétale, Faculté des Sciences et Techniques, Université Cheikh Anta Diop, Dakar, Sénégal 2Laboratoire de Microbiologie des sols ISRA-IRD, BP 1386, Dakar, Sénégal
Email: [email protected]

  •  Accepted: 15 January 2003
  •  Published: 28 February 2003

Abstract

 

Cowpea, Vigna unguiculata (L.) Walp. presents phenotypical variabilities and in order to study the genetic diversity of cultivated Senegalese varieties, two experimental approaches were used. First, a physiological characterization based on nitrogen fixation was used to assess cowpea breeding lines. Inoculation with two Bradyrhizobiumstrains (NGR234 and ISRA312), showed a difference in nitrogen fixation potential between the cowpea varieties. Diongoma is the highest nitrogen fixing variety, whereas Mouride is the lowest. The second approach employed genetic characterization based on DNA polymorphism to screen. Results suggest that random amplified polymorphic DNA (RAPD) technology can be used to reorganize the national germplasm in order to eliminate the putative duplicates, and to identify elite varieties.

 

Key words: Vigna unguiculata, nitrogen fixation, cowpea, molecular markers, RAPD.