African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12258

Full Length Research Paper

Impact of the application of humic acid and sodium nitroprusside on nickel toxicity: Analysis of relative gene expression

Sandra Perez Alvarez
  • Sandra Perez Alvarez
  • Productora Agricola ?El Encanto?, Guillermo Nelson y Cuauhtemoc, Sin numero altos, Dpto. 3, Colonia Centro, Guasave, Sinaloa, C.P. 81000 Mexico.
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Daniel Cabezas Montero
  • Daniel Cabezas Montero
  • Universidad Agraria de la Habana (UNAH) ?Fructuoso Rodriguez Perez?, Carretera Tapaste y Autopista Nacional, San Jose de las Lajas, Mayabeque, C. P.32700, Cuba.
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Norma Elena Leyva Lopez
  • Norma Elena Leyva Lopez
  • Instituto Politecnico Nacional, CIIDIR-IPN, Unidad Sinaloa, Departamento de Biotecnologia Agricola, Blvd. Juan de Dios Batiz Paredes No 250. Guasave, Sinaloa, C.P. 81101 Mexico.
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Jesus Mendez Lozano
  • Jesus Mendez Lozano
  • Instituto Politecnico Nacional, CIIDIR-IPN, Unidad Sinaloa, Departamento de Biotecnologia Agricola, Blvd. Juan de Dios Batiz Paredes No 250. Guasave, Sinaloa, C.P. 81101 Mexico.
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Esteban Sanchez Chavez*
  • Esteban Sanchez Chavez*
  • Centro de Investigacion en Alimentos y Desarrollo (CIAD), Unidad Delicias, Av. 4a. Sur 3820, Fracc. Vencedores del Desierto, Cd. Delicias, Chihuahua, C.P. 33089, Mexico.
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  •  Received: 18 June 2015
  •  Accepted: 17 August 2015
  •  Published: 26 August 2015

Abstract

Nickel (Ni) is an essential micronutrient for plants but in high concentrations may turn toxic. This paper discusses the potential role of humic acid (HA) and sodium nitroprusside in modulating or preventing oxidative stress in rice plants. Three genes [superoxide dismutase (SOD) glutathione reductase (GR) and ascorbate peroxidase (APx) were selected for an expression study using a real time PCR technique. Three different treatments (T1 = nickel [nickel chloride (NiCl2·6H2O)] 300 mg L-1, T2 = nickel-humic acid, T3 = nickel-sodium nitroprusside) were used to determine the effect of humic acid and sodium nitroprusside on nickel toxicity in rice plants. Rice plants grown in T2 appeared green and well developed. In leaves and roots, the expression of superoxide dismutase and ascorbate peroxidase was higher in T3 (nickel-sodium nitroprusside); glutathione reductase expression in roots was lower in T1 (sand with Ni solution) compared to T2 (nickel 300 mg L-1 and HA) where the expression was higher; significant differences were found between both treatments. In leaves, the behavior of this gene was similar in all treatments. This research suggests that nickel toxicity cannot be diminished when HA or SNP are used, and they induce oxidative stress in rice plants.

 

Key words: Nickel toxicity, heavy metals, gene expression, oxidative stress.

Abbreviation

HA, Humic acid; SOD, superoxide dismutase; GR, glutathione reductase; APx, ascorbate peroxidase; ROS, reactive oxygen species; CAT, catalase; SNP, sodium nitroprusside PCR, polymerase chain reaction.