African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12496

Full Length Research Paper

In vitro flowering in embryogenic cultures of Kinnow mandarin (Citrus nobilis Lour ´ C. deliciosa Tenora)

B. Singh1, S. Sharma1, G. Rani1, G.S. Virk1, A.A. Zaidi2 and A. Nagpal1*
1Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar 143005, India. 2Floriculture Division, Institute of Himalayan Bioresource Technology, C.S.I.R., Palampur 176 061 (H.P), India.
Email: [email protected]

  •  Accepted: 28 June 2006
  •  Published: 17 August 2006

Abstract

Embryogenic cultures of Kinnow mandarin (C. nobilis Lour × C. deliciosa Tenora) were raised from unfertilized ovules dissected from unopened flower buds of this plant inoculated on MS medium supplemented with 2 mg/L kinetin (KN). In vitro flowering was induced in these cultures by using different concentrations of KN and sucrose as well as subjecting these cultures to different photoperiods. Maximum percentage (31.94%) of cultures producing flowers and maximum number (5.58) of flowers per culture was observed on MS medium supplemented with KN (2 mg/L) and sucrose 40 g/L at 12-h photoperiod.

 

Key words: Embryogenic callus, in vitro flowering, Kinnow (Citrus nobilis ´ C. deliciosa), ovule culture.

Abbreviation

BA, 6‑benzyladenine; KN, kinetin; ME, malt extract; MS medium, Murashige and Skoog's (1962) medium

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This article is published under the terms of the Creative Commons Attribution License 4.0

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