Abstract

Ethanol-fixed entire bodies of the tsetse fly, Glossina fuscipes fuscipes, and unidentified stable flies, Stomoxys spp., collected from near Juba town, southern Sudan, were tested for Trypanozoon trypanosomes infections using polymerase chain reaction (PCR) technique for the first time in Sudan. The crude target DNA sequences were extracted by incubation of entire flies in Nonindet PCR template buffer containing proteinase-K. The DNA amplification sets of conditions were adjusted for each pair of primers employed. The oligonucleotide primers used included TBR_1-2, SRA_A-E, SRA_B537-B538 and TgsGP_FOR-REV. The results showed that 74.4% of G. f. fuscipes and 39.36% of Stomoxys spp. were infected withTrypanozoon trypanosomes. Out of the 117 examined G. f. fuscipes, 46.2, 24.8, 35.04, 17.09 and 10.26% were due to T. b. gambiense (TgsGP_FOR-REV), T. b. rhodesiense (SRA_A-E), T. b. rhodesiense (SRA_3537-3538), mixed infection with T. b. gambiense and T. b. rhodesiense and T. b. brucei, respectively. However, infections in Stomoxys spp. of 2.13 and 37.2% were due to T. b. rhodesiense andT. b. brucei, respectively.

Key words: Glossina fuscipes fuscipes, T. b. gambiense, T. b. Rhodesiense, vectoral capacity, infection rate, PCR technology.

Abbreviation

PCR, Polymerase chain reaction; HAT, human African trypanosomosis.