African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12191

Full Length Research Paper

Virus efficacy of recombined Autographa californica M nucleopolyhedrovirus (AcMNPV) on tea pest Ectropis obliqua

Yue-Rong Liang1, Chen Lin1, Xun-Lei Wang1, Jian-Hui Ye1, Jian-Liang Lu2*
1Zhejiang University Tea Research Institute, Hangzhou 310029, PR China. 2The State Agriculture Ministry Laboratory of Horticulture Plant Growth, Development and Quality Improvement, Hangzhou 310029, PR China.
Email: [email protected]

  •  Accepted: 16 July 2010
  •  Published: 31 August 2010

Abstract

Ectropis obliqua is a major tea pest and chitin synthase (CHS) plays a key role in the pest growth and development. A 192 bp conserved domain from E. obliqua CHS gene was cloned and it was used to construct recombined Autographa californicaM nucleopolyhedrovirus (AcMNPV) with double-stranded RNA interference (dsRNAi) method. The recombined AcMNPV virus could propagate in host cells sf9. Injection test showed that the virus efficacy of the recombined AcMNPV on E. obliqua larvae was significantly enhanced. It is considered that the CHS dsRNAi mediated by the nuclear polyhedrosis virus will be interesting for development of alternative bio-pesticide to control the tea pest E. obliqua.

 

Key words: Chitin synthase, baculovirus, double-stranded RNA interference,Ectropis obliqua.

Abbreviation

CHS, Chitin synthase; AcMNPV, Autographa californica M nucleopolyhedrovirus; dsRNAi, double-stranded RNA interference; RISC, RNA-induced silencing complex; PTGS, post-transcriptional gene silencing; NPV,nuclear polyhedrosis virus; RNAi, RNA interference.