African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12278

Full Length Research Paper

An improved plating assay for determination of phage titer

Liu Yang
  • Liu Yang
  • Key Laboratory of Pig Industry Sciences, Ministry of Agriculture, Rongchang, Chongqing 402460, China.
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Chao Li
  • Chao Li
  • Southwestern University Rongchang Campus, Rongchang, Chongqing 402460, China.
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Shao-Qin Zhai
  • Shao-Qin Zhai
  • Key Laboratory of Pig Industry Sciences, Ministry of Agriculture, Rongchang, Chongqing 402460, China.
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Hua Zheng
  • Hua Zheng
  • Key Laboratory of Pig Industry Sciences, Ministry of Agriculture, Rongchang, Chongqing 402460, China.
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Li-Zhi Fu
  • Li-Zhi Fu
  • Key Laboratory of Pig Industry Sciences, Ministry of Agriculture, Rongchang, Chongqing 402460, China.
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Da-Jun Li
  • Da-Jun Li
  • Key Laboratory of Pig Industry Sciences, Ministry of Agriculture, Rongchang, Chongqing 402460, China.
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  •  Received: 04 October 2015
  •  Accepted: 18 May 2016
  •  Published: 08 June 2016

Abstract

Phage is a virus that is parasitic on bacteria. It is very important to determine the titer of test sample in the study of phage. In this study, an improved plating assay was developed for detection of the number of recombinant phage Cap-T7 present in a test solution at a certain dilution point by counting the plaque forming units. The data demonstrated that the improved plating assay is fast, useful, and convenient for the determination of the phage titer in a sample.

Key words: Phage Cap-T7, detection method, plaque forming units.