African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12491

Full Length Research Paper

Evaluation of hygienic quality of ferment of local beer “dolo” used as condiment in Burkina Faso

MOGMENGA Iliassou
  • MOGMENGA Iliassou
  • Laboratory of Microbiology and Biotechnology, Research Center in Biological Food and Nutrition Sciences (CRSBAN), Department of Biochemistry and Microbiology, University Ouaga I Pr Josph KY-ZERBO, Burkina Faso.
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SOMDA Kounbèsiounè Marius
  • SOMDA Kounbèsiounè Marius
  • Laboratory of Microbiology and Biotechnology, Research Center in Biological Food and Nutrition Sciences (CRSBAN), Department of Biochemistry and Microbiology, University Ouaga I Pr Josph KY-ZERBO, Burkina Faso.
  • Google Scholar
KEITA Ibrahim
  • KEITA Ibrahim
  • Laboratory of Applied Molecular Biology, Faculty of Sciences and Techniques, University of Sciences, Techniques and Technologies of Bamako, BP: E 3206, Colline Badalabougou, Bamako, Mali.
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TRAORE Sababénédyo Alfred
  • TRAORE Sababénédyo Alfred
  • Laboratory of Microbiology and Biotechnology, Research Center in Biological Food and Nutrition Sciences (CRSBAN), Department of Biochemistry and Microbiology, University Ouaga I Pr Josph KY-ZERBO, Burkina Faso.
  • Google Scholar


  •  Received: 05 May 2017
  •  Accepted: 15 June 2017
  •  Published: 28 June 2017

Abstract

Rabilé is a ferment of local beer called “dolo” in Burkina Faso. It is used for local beer production and as condiment in sauces. Its process of production could be a source of contamination. So the quality of ferment needs to be guaranteed for consumers’ health. The aim of the study was to control the hygienic quality of local ferment consumed in Burkina Faso. A total of 70 samples were purchased in seven localities of Burkina Faso. The standard plate count method was used for microbial analysis. The results show that aerobic mesophilic flora varied from 8.34 ± 0.72 to 10.07 ± 0.51 log10 UFC.g-1; yeast and mold from 7.24 to 8.28 log10 UFC.g-1; Staphylococcus aureus from 4.08 to 4.76 log10 UFC.g-1; Enterococci from 3.47 to 4.61 log10 UFC.g-1 and total coliform from 2.12 to 2.32 log10 UFC.g-1. Salmonella spp. and Shiguella spp. were not detected in any of the samples. So, 100% of the samples from Ouahigouya were contaminated with sulphite reducing bacteria (BRS). All the samples analyzed were contaminated by S. aureus and Enterococci. The hygienic control of the ferment showed that the contamination caused by food borne pathogens can lead to food poisoning. Pearson correlation matrix of microorganisms’ distribution in the samples showed highest positive correlation between Enterococci and sulphite reducing bacteria at r = 0.888. Analysis of principal components exhibited the variability of microbial groups with 94.312% cumulative values of the variance and Eigen values ranging between 0.492 and 3.695. The data suggested that traditional beer ferments samples collected were not suitable in quality and there is need to ameliorate the process of production.

Key words: Local ferment, dolo, hygienic quality, food, Burkina Faso.