African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12357

Full Length Research Paper

Proteome level changes in the root of Brassica alboglabra induced by alachlor herbicide

Zuraifah Asrah Mohamad1,5, Daranee Chokchaichamnankit2, Kisana Bhinija3, N. Monique Paricharttanakul2, Jisnuson Svasti2,4, Pattana Srifah Huehne3 and Chantragan Srisomsap2*
1Applied Biological Sciences, Chulabhorn Graduate Institute, Bangkok 10210, Thailand. 2Laboratory of Biochemistry, Chulabhorn Research Institute, Bangkok 10210, Thailand. 3Laboratory of Biotechnology, Chulabhorn Research Institute, Bangkok 10210, Thailand. 4Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand. 5Institute for Medical Research, Kuala Lumpur, 50588, Malaysia.  
Email: [email protected]

  •  Accepted: 30 April 2013
  •  Published: 15 May 2013

Abstract

Chinese kale (Brassica alboglabra) is a famous and extensively grown vegetable in Southeast Asia. Despite its nutritional values, pesticides are heavily applied to it. In this study, changes in protein expression due to alachlor treatment on B. alboglabrawere investigated by using the 2-dimensional PAGE. Differential protein expressions were determined by using Image Master Software with volume (%) ≥2 fold as significant. Ten spots of interest have been identified by LC/MS/MS showing significant increases in differential protein expression between B. alboglabra roots treated with alachlor as compared to the untreated group which include HSC-cognate binding proteins, adenosylmethionine synthetase and beta-tubulin involved in defence mechanism in plants. Little is known about the function of other proteins identified which include knox-like proteins and hypothetical protein. Further investigations on plant proteomics would provide more information on the effects of different types of pesticides.

 

Key words: Brassica alboglabra, pesticides, proteomics, two-dimensional electrophoresis.

Abbreviation

2 DE, Two-dimensional electrophoresis; PAGE, polyacrylamide gel electrophoresis; LC/MS, liquid chromatography mass spectrometry; HPLC, high performance liquid chromatography.