African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12392

Full Length Research Paper

Development and validation of a high performance liquid chromatography-diode array detection (HPLC-DAD) method for the quantification of rutin and isoquercetin in Morus nigra L. (Moraceae)

Pedrita Alves Sampaio
  • Pedrita Alves Sampaio
  • Central de Análises de Fármacos, Medicamentos e Alimentos (CAFMA), Universidade Federal do Vale do São Francisco, 56.304-205, Petrolina, Pernambuco, Brazil.
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Grasielly Rocha Souza
  • Grasielly Rocha Souza
  • Universidade Federal do Piauí, 64049-550, Teresina, Piauí, Brazil.
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Pedro Guilherme Sousa de Sá
  • Pedro Guilherme Sousa de Sá
  • Central de Análises de Fármacos, Medicamentos e Alimentos (CAFMA), Universidade Federal do Vale do São Francisco, 56.304-205, Petrolina, Pernambuco, Brazil.
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Nathália Andrezza Carvalho de Souza
  • Nathália Andrezza Carvalho de Souza
  • Central de Análises de Fármacos, Medicamentos e Alimentos (CAFMA), Universidade Federal do Vale do São Francisco, 56.304-205, Petrolina, Pernambuco, Brazil.
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José Marcos Teixeira de Alencar Filho
  • José Marcos Teixeira de Alencar Filho
  • Central de Análises de Fármacos, Medicamentos e Alimentos (CAFMA), Universidade Federal do Vale do São Francisco, 56.304-205, Petrolina, Pernambuco, Brazil.
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Pedro José Rolim-Neto
  • Pedro José Rolim-Neto
  • Laboratório de Tecnologia dos Medicamentos (LTM), Universidade Federal de Pernambuco, 50.670-901, Recife, Pernambuco, Brazil.
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Jackson Roberto Guedes da Silva Almeida
  • Jackson Roberto Guedes da Silva Almeida
  • Núcleo de Estudos e Pesquisas de Plantas Medicinais (NEPLAME), Universidade Federal do Vale do São Francisco, 56.304-205, Petrolina, Pernambuco, Brazil.
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Larissa Araújo Rolim
  • Larissa Araújo Rolim
  • Laboratório de Tecnologia dos Medicamentos (LTM), Universidade Federal de Pernambuco, 50.670-901, Recife, Pernambuco, Brazil.
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  •  Received: 09 July 2018
  •  Accepted: 30 July 2018
  •  Published: 22 August 2018

Abstract

Morus nigra L. is widely used by the population of the São Francisco Valley (Brazil) for the treatment of several diseases such as hypertension, diabetes, arthritis etc. The objective of this study was to develop and validate an analytical method that is capable of identifing and quantifing flavonoids in the ethanolic crude extract (Mn-EtOH) and its ethyl acetate phase (Mn-AcOEt) obtained from M. nigra leaves using high performance liquid chromatography (HPLC). The plant material was collected and processed to obtain Mn-EtOH, which was subjected to a process of liquid/liquid partition, from which the AcOEt phase was obtained. The method was developed using an reverse phase (RP) C-18 column (250 × 4.6 mm, 5 μm) as the stationary phase. A mixture of acidified water (0.01% trifluoroacetic acid): acetonitrile was used as the mobile phase in a gradient system with a flow rate of 0.8 mL/min at a wavelength of 340 nm for the detection and determination. The method developed was considered to be specific: linear (R2 > 0.99), selective, accurate, and robust.

 

Key words: Flavonoids, isoquercetin, rutin, morus.