African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12258

Full Length Research Paper

Micropropagation of Launaea cornuta - an important indigenous vegetable and medicinal plant

Faith Ambajo
  • Faith Ambajo
  • Department of Biotechnology, University of Eldoret, P.O. Box 1125, Eldoret, Kenya.
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Jonathan Mutie Matheka
  • Jonathan Mutie Matheka
  • International Institute of Tropical Agriculture c/o ICIPE P.O. Box 30772-00100 Nairobi, Kenya.
  • Google Scholar


  •  Received: 06 January 2016
  •  Accepted: 19 July 2016
  •  Published: 10 August 2016

Abstract

An efficient micropropagation protocol was developed for the medicinal plant Launaea cornuta using green house axillary buds as explants. The best sterility was obtained at 30% (v/v) local bleach (JIK). Maximum shoot induction rate was achieved when axillary buds were cultured on Murashige and Skoog (MS) Media supplemented with 0.5 mg/L of 6-benzylaminopurine (BAP) for 3 weeks. The highest number of shoot multiplication was obtained when induced shoots were culture on MS media supplemented with 0.5 mg/L BAP and 0.2 mg/L NAA for 30 days. The best rooting response with regard to average root length, rooting percentage and number of roots was achieved within 4 weeks of culture of excised shoots on MS media having 0.5 mg/L BAP. Regenerated plants were successfully acclimatized and about 80 to 90% of plantlets survived under ex vitro conditions. About 170 plants were produced from a single nodal bud of L. cornuta after 60 days. A reproducible protocol was established for in vitro propagation of L. cornuta, an important indigenous vegetable with high medicinal value.

 

Key words: Launaea cornuta, tissue culture, micropropagation, axillary buds, tissue culture.