African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12191

Full Length Research Paper

Molecular marker screening of peanut (Arachis hypogaea L.) germplasm for Meloidogyne arenaria resistance

Valéria Carpentieri-Pípolo*
  • Valéria Carpentieri-Pípolo*
  • Agronomy Department, Universidade Estadual de Londrina, CP 6001, Londrina, PR, 86051-990, Brazil.
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Maria Gallo-Meagher
  • Maria Gallo-Meagher
  • University of Florida, IFAS, PO Box 110300, Gainesville, FL, 32611‑0300, USA.
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Don W. Dickson
  • Don W. Dickson
  • University of Florida, IFAS - 2212 Building 970, PO Box 110620, Gainesville, FL ,32611-0620, USA.
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Daniel W. Gorbet2,
  • Daniel W. Gorbet2,
  • University of Florida, IFAS, PO Box 110300, Gainesville, FL, 32611‑0300, USA.
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Maria De Lurdes Mendes
  • Maria De Lurdes Mendes
  • University of Florida, IFAS - 2212 Building 970, PO Box 110620, Gainesville, FL, 32611-0620, USA.
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Silvia Graciele Hülse de Souza
  • Silvia Graciele Hülse de Souza
  • 5Laboratory of Molecular Biology, Universidade Paranaense, CP224, Umuarama, 87502-210, Brazil.
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  •  Received: 25 March 2014
  •  Accepted: 02 June 2014
  •  Published: 25 June 2014

Abstract

Meloidogyne arenaria (Neal) Chitwood race 1, along with visual evaluation following root staining were used to screen four breeding populations and three lines of peanut (Arachis hypogaea L.) in a root-knot nematode infested field. COAN and Florunner peanut cultivars were used as resistant and susceptible controls, respectively. Genomic DNA was isolated from young leaves of plants during the growing season, and Southern blot analysis was conducted using RFLP probe R2430E. Only COAN and the line TP301-1-8 were homozygous for the resistance marker. During evaluation, root masses were counted and the resistance phenotype scored. This field data confirmed the RFLP marker results. Except for COAN and TP301-1-8, all other genotypes displayed high levels of nematode reproduction. The RFLP probe R2430E provided a useful marker for identifying resistance to the peanut root-knot nematode.

 

Key words: Arachis hypogaea L., genetics, host resistance, Meloidogyne spp., molecular markers, peanut root-knot nematode.

Abbreviation

Abbreviation: RFLP, Restriction fragment length polymorphism.