African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12269

Full Length Research Paper

Genetic diversity of grape germplasm as revealed by microsatellite (SSR) markers

Lei Wang
  • Lei Wang
  • School of Life Science, Ludong University, Yantai 264025, Shandong, China.
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Juan Zhang*
  • Juan Zhang*
  • School of Agriculture, Ludong University, Yantai 264025, Shandong, China.
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Linde Liu
  • Linde Liu
  • School of Life Science, Ludong University, Yantai 264025, Shandong, China.
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Li Zhang
  • Li Zhang
  • School of Life Science, Ludong University, Yantai 264025, Shandong, China.
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Lijuan Wei
  • Lijuan Wei
  • School of Life Science, Ludong University, Yantai 264025, Shandong, China.
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Dechang Hu
  • Dechang Hu
  • School of Life Science, Ludong University, Yantai 264025, Shandong, China.
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  •  Received: 10 September 2014
  •  Accepted: 17 March 2015
  •  Published: 25 March 2015

Abstract

In this work, cluster analysis and principal component analysis (PCA) were used to study the genetic diversity and relationships among 49 grape germplasm accessions analyzed with 19 simple sequence repeat (SSR) primer pairs. In total, 139 polymorphic loci were detected among these accessions with an average of 7.32 polymorphic loci per SSR primer pair. The average values for the effective number of alleles, Nei’s gene diversity, and Shannon’s information index were 1.5605, 0.3352 and 0.5064, respectively. The cluster analysis showed that the 49 accessions could be divided into five groups and an outgroup. The results of the PCA were nearly consistent with those of unweighted pair-group method with arithmetic averages (UPGMA) clustering analysis. These results will be useful for the exploitation of grape germplasm in basic and applied research.
 
Key words: Vitis vinifera L., simple sequence repeat (SSR), genetic diversity, principal component analysis. 
 

Abbreviation

PCA, principal component analysis; SSR, simple sequence repeat; UPGMA, unweighted pair-group method with arithmetic averages.