African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12296

Full Length Research Paper

Influence of sweeteners in the biodistribution of radiopharmaceutical and laboratory tests in rats

Michelly Pires Queiroz
  • Michelly Pires Queiroz
  • Postgraduate Program in Science and Technology of Food, Federal University of Paraiba, Brazil.
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Vanessa Santos de Arruda Barbosa
  • Vanessa Santos de Arruda Barbosa
  • Center for Education and Health, Federal University of Campina Grande, Cuité, Paraíba, Brazil.
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Cecília Maria de Carvalho Xavier Holanda
  • Cecília Maria de Carvalho Xavier Holanda
  • Department of Microbiology and Parasitology, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.
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Janette Monroy Osorio
  • Janette Monroy Osorio
  • Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.
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Tarciso Bruno Montenegro Sampaio
  • Tarciso Bruno Montenegro Sampaio
  • Postgraduate Program in Biotechnology, Potiguar University, Natal, Rio Grande do Norte, Brazil.
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Christina da Silva Camillo
  • Christina da Silva Camillo
  • Department of Morphology, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.
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Aldo Cunha Medeiros
  • Aldo Cunha Medeiros
  • Department of Surgery, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.
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Marília Ferreira Frazão Tavares de Melo
  • Marília Ferreira Frazão Tavares de Melo
  • Center for Education and Health, Laboratory of Experimental Nutrition, Federal University of Campina Grande, Cuité, Paraíba, Brazil.
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Juliana Késsia Barbosa Soares
  • Juliana Késsia Barbosa Soares
  • Center for Education and Health, Laboratory of Experimental Nutrition, Federal University of Campina Grande, Cuité, Paraíba, Brazil.
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  •  Received: 05 January 2016
  •  Accepted: 25 July 2016
  •  Published: 14 December 2016

Abstract

This study aimed to evaluate the effect of supplementation of sucralose and fructose on the metabolism of adolescent rats. Eighteen male Wistar rats were divided into 3 groups: control group (GC), fructose group (GF) treated with 50 mg/kg of fructose, and sucralose group (GS) receiving 50 mg/kg of sucralose for 24 days. The weight and feed intake were measured weekly. At the end of the experiment, some biochemical parameters, histopathology of the liver and biodistribution of the radiotracer 99mTc-sodium phytate in liver and blood were analyzed. The GF showed higher body weight only in the first week compared with GS and GC (p<0.05). Histopathology and % ATI/g radiotracer 99mTc-sodium phytate in liver and blood were not different between the groups. The GF showed higher values ​​of aspartate aminotransferase activity, bilirubin, alkaline phosphatase activity and gamma glutamyl transferase activity, compared with the other groups (GC and GS) (p<0.05). Activity of alanine aminotransferase and albumin level of GF were higher than GS (p<0.05). For other parameters, no statistical difference was observed. It was concluded that the use of fructose during the experiment was able to alter hepatic enzymes, but on the other hands, the use of sucralose caused no change.

 

Key words: Sucralose, fructose, adolescent rats, radiopharmaceutical.