African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12191

Full Length Research Paper

Occurrence of Bacillus thuringiensis in faeces of herbivorous farm animals

  S. Maheswaran1, S. Sreeramanan2*, C.M. Reena Josephine1, K. Marimuthu1 and R. Xavier1  
1Department of Biotechnology, Faculty of Applied Sciences, AIMST University, Semeling Kedah, Malaysia. 2School of Biological Sciences, Universiti Sains Malaysia, Penang, Malaysia.  
Email: [email protected]

  •  Accepted: 08 October 2010
  •  Published: 22 November 2010

Abstract

 

Bacillus thuringiensis (Berliner), the insect pathogen has been isolated from a variety of habitat. It is understood that the habitat of B. thuringiensis has always been associated with their biological activity. In the present study, B. thuringiensiswas isolated from faeces of cows and goats. The phenotypic characterization revealed that the B. thuringiensis isolates are motile and harbour bipyramidal and spherical crystals, which was confirmed by the electron microscopic studies. The biochemical characterization confirmed the haemolytic nature and nitrate reduction ability of the B. thuringiensis isolates. There is a distinct variation in protein profiles among the isolates as revealed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis. From this study we hypothesize that application of cattle manure in addition to maintaining the soil health also help augment B. thuringiensis population in soil, as a natural biocontrol agent and may play a role in the biological nitrogen cycle.

 

Key words: Bacillus thuringiensis, isolation, animal faces.

Abbreviation

Abbreviations: SDS-PAGE, Sodium dodecyl sulfate-polyacrylamide gel electrophoresis; Bt, Bacillus thuringiensisFM, farmyard manure; CBB, Coomassie brilliant blue.