African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12193

Full Length Research Paper

Effect of extracts of Trichilia silvatica C. DC., on development and reproduction parameters of Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae)

Alessandra F. Freitas*
  • Alessandra F. Freitas*
  • Universidade Federal da Grande Dourados (UFGD), Faculdade de Ciências Biológicas e Ambientais (FCBA), Dourados-MS, Brazil.
  • Google Scholar
Anelise S. N. Formagio,
  • Anelise S. N. Formagio,
  • Universidade Federal da Grande Dourados (UFGD Faculdade de Ciências Agrárias (FCA), Dourados-MS, Brazil
  • Google Scholar
Fabricio F. Pereira
  • Fabricio F. Pereira
  • Universidade Federal da Grande Dourados (UFGD), Faculdade de Ciências Biológicas e Ambientais (FCBA), Dourados-MS, Brazil.
  • Google Scholar
Jéssica T. Lucchetta
  • Jéssica T. Lucchetta
  • Universidade Federal da Grande Dourados (UFGD), Faculdade de Ciências Biológicas e Ambientais (FCBA), Dourados-MS, Brazil.
  • Google Scholar
Maria Do Carmo Vieira
  • Maria Do Carmo Vieira
  • Universidade Federal da Grande Dourados (UFGD Faculdade de Ciências Agrárias (FCA), Dourados-MS, Brazil
  • Google Scholar


  •  Received: 01 March 2014
  •  Accepted: 30 April 2014
  •  Published: 14 May 2014

Abstract

The objective of this study was to evaluate the effects of the methanolic extracts from the leaves, bark and flowers of Trichilia silvatica on Spodoptera frugiperda. Also, it was use in evaluating the total phenolic and flavonoid content of methanolic extracts. We also reported chemical study on the most active extract. Corn leaves were immersed in a 1% methanolic extract solution and fed to second instars of S. frugiperda. The extract of the T. silvatica (LTS) leaves decreased the viability of the larva, prolonged larval duration, affected the pupal biomass, decreased the period of oviposition and the number of eggs as well as affected the egg viability. The methanolic extract of the T. silvatica (BTS) bark decreased the larval viability, oviposition period, number of eggs and egg viability. The flower extract of T. silvatica (FTS) decreased the larval viability and period of oviposition. In relation to the constituent contents, the methanolic extract of the leaves showed highest total phenol (233.37 mg gallic acid/g of extract) and flavonoid (53.17 mg quercetin/g of extract) content. The chemical study of the FTS resulted in α-tocopherol, sitosterol 3-O-glucopyranoside, mustakone and N-metilproline. Our results indicate that the extracts affected the biology of S. frugiperda, with LTS being the most promising.

 

Key words: Meliaceae, methanolic extracts, insecticides, plant-derived compounds.

Abbreviation

Abbreviations: LTS, Leaves of Trichilia silvatica; BTS, bark of Trichilia silvatica; FTS, flower of Trichilia silvatica.