African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12496

Full Length Research Paper

Identification and molecular characterization of Caryedon furcatus (Anton & Delobel), the main pest of Senegalia macrostachya (Reichenb. ex DC. Kyal & Boatwr) seeds in storage in Burkina Faso

Larissa Dioma
  • Larissa Dioma
  • Laboratoire d’Entomologie Fondamentale et Appliquée, Université Joseph KI ZERBO, 06 BP 9499 Ouagadougou 06, Burkina Faso.
  • Google Scholar
Jean Christophe Koussoubé
  • Jean Christophe Koussoubé
  • Laboratoire d’Entomologie Fondamentale et Appliquée, Université Joseph KI ZERBO, 06 BP 9499 Ouagadougou 06, Burkina Faso.
  • Google Scholar
Mbacké Sembène
  • Mbacké Sembène
  • Département de Biologie Animale, Faculté des Sciences et Techniques, Université Cheikh Anta Diop, B.P. 5005 Dakar, Sénégal.
  • Google Scholar
Antoine Sanon
  • Antoine Sanon
  • Laboratoire d’Entomologie Fondamentale et Appliquée, Université Joseph KI ZERBO, 06 BP 9499 Ouagadougou 06, Burkina Faso.
  • Google Scholar
Zakaria Ilboudo
  • Zakaria Ilboudo
  • Laboratoire d’Entomologie Fondamentale et Appliquée, Université Joseph KI ZERBO, 06 BP 9499 Ouagadougou 06, Burkina Faso.
  • Google Scholar


  •  Received: 02 August 2024
  •  Accepted: 13 September 2024
  •  Published: 31 October 2024

Abstract

In Burkina Faso, seeds of Senegalia macrostachya (Rchb. Ex DC.) Kyal. & Boatwr. during storage are attacked by a pest morphologically identified as Caryedon mauritanicus Auct. It was described for the first time in 2004 under a new name 'Caryedon furcatus' by Anton and Delobel. Thus, C. mauritanicus is now considered to be C. furcatus. In the present study, for the first time, genetic characterization of C. furcatus was carried out to determine its genetic identity in order to assess whether it is evolutionarily similar or related to C. mauritanicus. The pest's 12S gene was partially sequenced after extraction and amplification by Polymerase Chain Reaction. The percentage identity and variability of genetic parameters between C. furcatus and C. mauritanicus sequences were determined. A very high percentage of identity, ranging from 93.10 to 99.67% with respect to the C. mauritanicus species was obtained. The results also showed that eleven of the 208 sites were polymorphic. The values for nucleotide composition show a predominance of Adenine and Thymine (76.32%) when compared with Guanine and Cytosine (23.68%). C. furcatus has high haplotypic diversity (0.717) and low nucleotide diversity (0.006). The similarity percentage obtained shows that C. furcatus is genetically identical to C. mauritanicus.

 

Key words: Senegalia macrostachya, Caryedon furcatus, Caryedon mauritanicus, molecular identification, genetic identity.