Full Length Research Paper
Abstract
In Burkina Faso, seeds of Senegalia macrostachya (Rchb. Ex DC.) Kyal. & Boatwr. during storage are attacked by a pest morphologically identified as Caryedon mauritanicus Auct. It was described for the first time in 2004 under a new name 'Caryedon furcatus' by Anton and Delobel. Thus, C. mauritanicus is now considered to be C. furcatus. In the present study, for the first time, genetic characterization of C. furcatus was carried out to determine its genetic identity in order to assess whether it is evolutionarily similar or related to C. mauritanicus. The pest's 12S gene was partially sequenced after extraction and amplification by Polymerase Chain Reaction. The percentage identity and variability of genetic parameters between C. furcatus and C. mauritanicus sequences were determined. A very high percentage of identity, ranging from 93.10 to 99.67% with respect to the C. mauritanicus species was obtained. The results also showed that eleven of the 208 sites were polymorphic. The values for nucleotide composition show a predominance of Adenine and Thymine (76.32%) when compared with Guanine and Cytosine (23.68%). C. furcatus has high haplotypic diversity (0.717) and low nucleotide diversity (0.006). The similarity percentage obtained shows that C. furcatus is genetically identical to C. mauritanicus.
Key words: Senegalia macrostachya, Caryedon furcatus, Caryedon mauritanicus, molecular identification, genetic identity.
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