African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12500

Full Length Research Paper

Phytotoxic characterization of crude methanolic extract of Periploca aphylla

Rahmat Ali Khan1*, Noor Aslam Khan1, Mustaq Ahmed1, Muhammad Rashid Khan2, Farid Ullah Khan1, Abdus Saboor Shah1 and Mir Sadiq Shah1        
1Department of Biotechnology, Faculty of Biological Sciences, University of Science and Technology, Bannu, Khyber Pakhtunkhwa, Pakistan. 2Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
Email: [email protected]

  •  Accepted: 28 May 2012
  •  Published: 03 July 2012

Abstract

Periploca aphylla is traditionally used in the treatment of various ailments. Phytotoxic activity of crude methanolic extract of P. aphylla was tested on the germination of wheat seeds and on the growth of the germinated seedlings. In both the field and plate studies, the extract showed inhibitory effect on the germination of the growth of root and shoot of the seedlings. The inhibition was found to be dose dependent. The higher concentration of 1000 μg/ml showed maximum inhibitory effect on the growth of root and shoots in the studies of plate as well as on fresh and dry weight of wheat plant. Similarly, the herbicidal activity is also dependent on the concentration of extract. In this study, it was found that inhibitory potential of methanolic extract of P. aphyllaincreased as 1000 >100 >10 μg/ml.

 

Key words: Phytotoxic activity, herbicidal activity, germination, plates and field studies, Perifloca aphylla.