African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12254

Full Length Research Paper

Production and characterization of β-glucosidase from Gongronella butleri by solid-state fermentation

Flávia Regina da Silva Santos
  • Flávia Regina da Silva Santos
  • Laboratory of Enzymology and Fermentation Processes, Faculty of Biological and Environmental Sciences, Federal University of Grande Dourados (FCBA/UFGD), Dourados - MS, Brazil.
  • Google Scholar
Nayara Fernanda Lisboa Garcia
  • Nayara Fernanda Lisboa Garcia
  • Laboratory of Enzymology and Fermentation Processes, Faculty of Biological and Environmental Sciences, Federal University of Grande Dourados (FCBA/UFGD), Dourados - MS, Brazil.
  • Google Scholar
Marcelo Fossa da Paz
  • Marcelo Fossa da Paz
  • Laboratory of Enzymology and Fermentation Processes, Faculty of Biological and Environmental Sciences, Federal University of Grande Dourados (FCBA/UFGD), Dourados - MS, Brazil.
  • Google Scholar
Gustavo Graciano Fonseca
  • Gustavo Graciano Fonseca
  • Laboratory of Bioengineering, Faculty of Biological and Environmental Sciences, Federal University of Grande Dourados (FCBA/UFGD), Dourados - MS, Brazil.
  • Google Scholar
Rodrigo Simões Ribeiro Leite
  • Rodrigo Simões Ribeiro Leite
  • Laboratory of Enzymology and Fermentation Processes, Faculty of Biological and Environmental Sciences, Federal University of Grande Dourados (FCBA/UFGD), Dourados - MS, Brazil.
  • Google Scholar


  •  Received: 05 October 2015
  •  Accepted: 11 March 2016
  •  Published: 20 April 2016

Abstract

Among the enzymes of the cellulolytic complex, β-glucosidases are noteworthy due to the possibility of their application in different industrial processes, such as production of biofuels, winemaking, and development of functional foods. This study aimed to evaluate the production and characterization of β-glucosidase from the filamentous fungus Gongronella butleri, recently isolated from Cerrado soil and cultivated in agro-industrial residue substrates. The highest production of β-glucosidase, about 215.4 U/g of dry substrate (or 21.5 U/mL), was obtained by cultivation of the microorganism on wheat bran with 55% of the initial moisture, for 96 h at 30°C. This β-glucosidase showed higher catalytic activity at pH 4.5, and a temperature of 65°C. The original enzymatic activity was recovered in a pH range of 3.0-7.5 after 24 h of incubation. The enzyme retained 80% of its catalytic activity when incubated for 1 h at 50°C. The enzyme was strongly inhibited by glucose, an effect that was completely reversed by increasing substrate concentration in the reaction mixture, which is typical for competitive inhibition. High catalytic activity was observed in solutions containing up to 20% ethanol, allowing the application of this enzyme in processes with high alcohol concentrations (for example beverages and biofuels). The significant production of β-glucosidase by the selected strain, along with these enzyme characteristics, highlights the biotechnological potential of the fungus G. butleri.

Key words: Microbial enzyme, biofuels, agro-industrial residues, cellulases, hemicellulases.