African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12302

Full Length Research Paper

Yield coefficient for the growth of Pseudomonas sp. AQ5-04 at various concentrations of phenol

Aisami A.
  • Aisami A.
  • Department of Biochemistry, Faculty of Sciences, Gombe State University, P. M. B. 027, Gombe, Nigeria.
  • Google Scholar
Usman M.M.
  • Usman M.M.
  • Department of Biotechnology, School of Life Science, Modibbo Adama University of Technology, Yola, Nigeria.
  • Google Scholar
Siddan A.A.
  • Siddan A.A.
  • Department of Biochemistry, Faculty of Sciences, Gombe State University, P. M. B. 027, Gombe, Nigeria.
  • Google Scholar
Ramlatu M.A.
  • Ramlatu M.A.
  • Department of Biochemistry, Faculty of Sciences, Gombe State University, P. M. B. 027, Gombe, Nigeria.
  • Google Scholar
James I.J.
  • James I.J.
  • Department of Biochemistry, Faculty of Sciences, Gombe State University, P. M. B. 027, Gombe, Nigeria.
  • Google Scholar
Garba L.
  • Garba L.
  • Department of Microbiology, Faculty of Science, Gombe State University, P. M. B. 027, Gombe, Nigeria.
  • Google Scholar
Yasid N.A.
  • Yasid N.A.
  • Department of Biochemistry, Faculty of Biotechnology and Bimolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
  • Google Scholar
Shukor M.Y.
  • Shukor M.Y.
  • Department of Biochemistry, Faculty of Biotechnology and Bimolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
  • Google Scholar


  •  Received: 19 February 2020
  •  Accepted: 20 March 2020
  •  Published: 31 July 2020

Abstract

Phenol is widely used by many industries in Malaysia, and is one of the highly toxic environmental pollutants with levels exceeding the regulatory standard that have been reported in Malaysia. A phenol-degrading bacterium; Pseudomonas sp. AQ5-04, a metabolically versatile xenobiotic-degrading bacterium, has been isolated from a local contaminated site. The bacterium was able to withstand a high concentration of phenol. This work aims to determine the effect of concentration and to establish the yield factor on phenol as a carbon source. It was established that the isolate can degrade up to 900 mg/L phenol within 72 h; it was discovered that the yield factor (YX/S) varied between 0.3 to 0.6 mg cells per mg phenol for different initial phenol concentrations with increasing phenol concentrations resulting in a lower yield. This shows that growth on phenol is strongly inhibitory and resulted in low yield at an inhibitory concentration of phenol.

 

Key words: Pseudomonas sp. AQ5-04, yield factor, phenol, inhibitory concentration.