African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12501

Full Length Research Paper

Detection of genetic variation in sample of Iranian proofed Holstein cattle by using microsatellite marker

Mohammad Reza Movahedin1, Cyrus Amirinia2*, Alireza Noshary1 and S. Ahmad Mirhadi2
  1Department of Animal Science, Karaj Branch, Islamic Azad University, Karaj, Mehr Shahr, Iran. 2Department of Biotechnology, Animal Science Research Institute of Iran, Karaj, Iran.
Email: [email protected]

  •  Accepted: 22 October 2010
  •  Published: 27 December 2010

Abstract

 

This study describes genetic variation among samples of Iranian Holstein cattle (Bos taurus) by using microsatellite markers. Semen samples of individuals were taken followed by DNA extraction. A panel of 13 microsatellites was used for evaluation of 13 loci in 68 Holstein proofed bulls. Mean value for allele per locus detected is 6.615, ranging from 10 (SPS115) to 4 (ETH3). All the microsatellite DNA markers showed high polymorphism and displayed a relatively high level of genetic variation as estimated by allelic diversity and heterozygosity. Estimated heterozygosities ranged from 1.000 (BM2113, TGLA122, TGLA126, ETH3, MGTG4B, SPS115, TGLA227 and INRAO23) to 0.633 (SPS113) with mean value of 0.946. All the loci showed deviation from Hardy- Weinberg equilibrium (p < 0.001), polymorphism information content (PIC) calculated for each marker exceeded 0.6 and the mean value of Shannon information index was estimated to be 1.606. Obtained results showing heterozygousity can be useful for the development of breeding strategies for genetic improvement in Iranian Holstein cattle.

 

Key words: Genetic variability, polymorphism, microsatellite loci, Bos taurus, Iranian Holstein cattle, DNA.