African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12209

Full Length Research Paper

Expression of porcine myosin heavy chain 1 gene in Berkshire loins with a high pH24 value

  Jin Hun Kang1#, Woo Young Bang1#, Eun Jung Kwon1, Yong Hwa Lee1, Da Hye Park1, Eun Seok Cho1, Min Ji Kim1, Jong-Soon Choi1,2, Hwa Chun Park3, Beom Young Park4 and Chul Wook Kim1*  
  1Swine Science and Technology Center, Jinju National University, Jinju 660-758, Korea. 2Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon 305-764, Korea 3Pig Breeding Company, Namwon 590-831, Korea. 4National Institute of Animal Science, RDA, Suwon 441-350, Korea.  
Email: [email protected]

  •  Accepted: 04 October 2010
  •  Published: 01 November 2010

Abstract

 

An important factor in evaluating porcine meat quality is the pH at post-mortem 24 h (pH24). In the current work, we conducted a GeneFishing polymerase chain reaction (PCR)–based screen to identify differentially expressed genes in Berkshire loins with high versus low pH24. Total RNAs were extracted from Berkshire loins with high and low pH24 and subjected to GeneFishing PCR. Four genes were found to be differentially expressed in the high and low pH24 groups. Semi-quantitative reverse transcription (RT)-PCR confirmed that MYH1 encoding myosin heavy chain 1 was specifically expressed in high pH24 groups. These results are very useful in establishing the genetic relationship between the composition of muscle fiber type and pH24 in the Berkshire loin.

 

Key words: GeneFishingTM polymerase chain reaction, myosin heavy chain 1, pork meat quality, post-mortem pH value, pale, soft and exudative.

Abbreviation

Abbreviations: ACP, Annealing control primer; pH24, pH at post-mortem 24 h; ATP, adenosine triphosphate; DEG, differentially expressed gene; MYH1, myosin heavy chain 1; PCR, polymerase chain reaction; RT-PCR, reverse transcription-PCR; PSE, pale, soft and exudative; RT, reverse transcription.