African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12300

Full Length Research Paper

Silver nanoparticles biosynthesized by secondary metabolites from Moringa oleifera stem and their antimicrobial properties

Eric Cristina Arrieta
  • Eric Cristina Arrieta
  • Instituto de Ingeniería, Universidad Autónoma de Baja California (UABC), Calle de la Normal s/n y Boulevard Benito Juárez, 21100 Mexicali, Baja California, México.
  • Google Scholar
Benjamín Valdez*
  • Benjamín Valdez*
  • Instituto de Ingeniería, Universidad Autónoma de Baja California (UABC), Calle de la Normal s/n y Boulevard Benito Juárez, 21100 Mexicali, Baja California, México.
  • Google Scholar
Monica Carrillo
  • Monica Carrillo
  • Instituto de Ingeniería, Universidad Autónoma de Baja California (UABC), Calle de la Normal s/n y Boulevard Benito Juárez, 21100 Mexicali, Baja California, México.
  • Google Scholar
Mario Alberto Curiel
  • Mario Alberto Curiel
  • Instituto de Ingeniería, Universidad Autónoma de Baja California (UABC), Calle de la Normal s/n y Boulevard Benito Juárez, 21100 Mexicali, Baja California, México.
  • Google Scholar
Francisco David Mateos
  • Francisco David Mateos
  • Instituto de Ingeniería, Universidad Autónoma de Baja California (UABC), Calle de la Normal s/n y Boulevard Benito Juárez, 21100 Mexicali, Baja California, México.
  • Google Scholar
Rogelio Arturo Ramos
  • Rogelio Arturo Ramos
  • Instituto de Ingeniería, Universidad Autónoma de Baja California (UABC), Calle de la Normal s/n y Boulevard Benito Juárez, 21100 Mexicali, Baja California, México.
  • Google Scholar
Navor Rosas
  • Navor Rosas
  • Instituto de Ingeniería, Universidad Autónoma de Baja California (UABC), Calle de la Normal s/n y Boulevard Benito Juárez, 21100 Mexicali, Baja California, México.
  • Google Scholar
Jose Maria Bastidas
  • Jose Maria Bastidas
  • Instituto de Ingeniería, Universidad Autónoma de Baja California (UABC), Calle de la Normal s/n y Boulevard Benito Juárez, 21100 Mexicali, Baja California, México.
  • Google Scholar


  •  Received: 16 December 2016
  •  Accepted: 10 February 2017
  •  Published: 01 March 2017

Abstract

Biological extracts have recently shown a great potential for rapid biosynthesis of silver nanoparticles (AgNPs) with antibacterial activity. AgNPs were synthetized by reduction effect of secondary metabolites produced by the Nigrospora sp. fungus, from Moringa oleifera stem as a reducing agent and silver nitrate (AgNO3) (1 mM) as salt precursor. The synthesis of nanoparticles (NPs) was monitored through analysis of the UV-Vis spectroscopy absorption in the 436 to 440 nm range indicating the presence of AgNPs in the colloidal aqueous solutions. Fourier transform infrared spectroscopy (FTIR) spectra were performed to identify the compounds responsible for the bio reduction of the Ag+. The morphology and sizes of AgNPs were characterized by scanning electron microscopy (SEM), energy dispersive of X-ray spectroscopy (EDS), dynamic light scattering (DLS), and colloid stability by zeta potential measurements. The NPs obtained were spherical in shape with size in the 3 to 70 nm range. Antibacterial activity was confirmed by evaluation of their effect against Escherichia coli, Klebsiella cloacae and Staphylococcus epidermidis. The proposed green synthesis of AgNPs from secondary metabolites produced by the Nigrospora sp. fungus from M. oleifera stem can be strongly recommended as a potential method for biomedical application.

Key words: Silver nanoparticles, secondary metabolites, green chemistry, antibacterial activity.