African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12278

Full Length Research Paper

Flow cytometry approach for studying the interaction between Bacillus mojavensis and Alternaria alternata

Asma MILET*
  • Asma MILET*
  • Laboratory of Mycology, Biotechnology and Antimicrobial Activity, Department of Microbiology, University Mentouri of Constantine, Ain Elbey, Constantine 25017, Algeria.
  • Google Scholar
Noreddine Kacem CHAOUCHE
  • Noreddine Kacem CHAOUCHE
  • Laboratory of Mycology, Biotechnology and Antimicrobial Activity, Department of Microbiology, University Mentouri of Constantine, Ain Elbey, Constantine 25017, Algeria.
  • Google Scholar
Laid DEHIMAT
  • Laid DEHIMAT
  • Laboratory of Mycology, Biotechnology and Antimicrobial Activity, Department of Microbiology, University Mentouri of Constantine, Ain Elbey, Constantine 25017, Algeria.
  • Google Scholar
Asma Ait KAKI
  • Asma Ait KAKI
  • Laboratory of Mycology, Biotechnology and Antimicrobial Activity, Department of Microbiology, University Mentouri of Constantine, Ain Elbey, Constantine 25017, Algeria.
  • Google Scholar
Mounira Kara ALI
  • Mounira Kara ALI
  • Laboratory of Mycology, Biotechnology and Antimicrobial Activity, Department of Microbiology, University Mentouri of Constantine, Ain Elbey, Constantine 25017, Algeria.
  • Google Scholar
Philippe THONART
  • Philippe THONART
  • Wallon Center of Industrial Biology, Agro-Bio Tech, University of Liege Gembloux, 5030, Belgium.
  • Google Scholar


  •  Received: 26 November 2015
  •  Accepted: 10 June 2016
  •  Published: 29 June 2016

Abstract

Tomato, Solanum lycopersicum is one of the most important vegetable crops consumed in Algeria. Tomato crops are often infected by Alternaria alternata, which causes early blight disease. Chemical pesticides are intensively used to protect this plant, which lead to environmental pollution that might endanger animal and human health. The main objective of this study is to select potential biocontrol agents from arid soil as an alternative to chemical products. The phytopathogenic fungus which was isolated from infested tomato leaves, stems and fruits cultured in Constantine-Algeria, was identified as Alternaria cf. alternata. Thirty five bacteria isolates were obtained from arid soil in the south of Algeria. Three of the isolates inhibited the growth of A. alternata. However, the most potent isolate, E1B3 reached a 75% inhibition rate. The molecular identification of this isolate showed that it was closely related to Bacillus mojavensis (KC977492). This strain does not produce chitinase, but does produce lipase, protease and lipopeptides. The interaction between A. alternata and B. mojavensis was investigated for the first time in this work by flow cytometric analysis. In conclusion, B. mojavensis strain was antagonistic to A. alternata which could possibly be exploited as a biopesticide in tomato crops management.

Key words: Tomato, Bacillus mojavensis, early blight, Alternaria alternata, flow cytometry.