African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12269

Full Length Research Paper

Effect of alkaline treatment on the sulfate content and quality of semi-refined carrageenan prepared from seaweed Kappaphycus alvarezii Doty (Doty) farmed in Indian waters

J. Moses
  • J. Moses
  • Research and Development Division Aquagri Processing Private Limited B5, SIPCOT Industrial Complex Manamadurai - 630 606, Sivaganga District Tamil Nadu, India.
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R. Anandhakumar
  • R. Anandhakumar
  • Research and Development Division Aquagri Processing Private Limited B5, SIPCOT Industrial Complex Manamadurai - 630 606, Sivaganga District Tamil Nadu, India.
  • Google Scholar
M. Shanmugam*
  • M. Shanmugam*
  • Research and Development Division Aquagri Processing Private Limited B5, SIPCOT Industrial Complex Manamadurai - 630 606, Sivaganga District Tamil Nadu, India.
  • Google Scholar


  •  Accepted: 22 April 2015
  •  Published: 06 May 2015

Abstract

Seaweed Kappaphycus alvarezii previously known as Eucheuma cottonii is one of the best sources of kappa carrageenan and is cultivated in Philippines, Indonesia, Malaysia and other countries including India. In the present study, semi-refined carrageenan (SRC) was prepared from K. alvarezii with different concentrations of KOH (6, 12, 18 and 24%) at 80 ± 2°C for 2 h; its sulfate contents were 13.73±0.74, 13.66±0.27, 13.84±0.66 and 14.76±0.18%, respectively, and it was 14.10±0.34% for untreated clean weed (control). The sulfate removal with increasing concentration of KOH used for processing SRC is not statistically significant (p < 0.05). The KCl gel strength of untreated clean weed and 6, 12, 18 and 24% KOH treated weed was 97±10.27, 650±12.11, 637±10.25, 552±18.71 and 526±26.55 g cm-2, respectively. Contents of 3,6-anhydrogalactose were 28.30±0.52, 33.41±0.50, 32.97±0.42, 31.15±0.60 and 31.61±0.17%. FTIR spectroscopy showed that molecules in all four SRC samples are quite similar. Spectral band was at 1257 cm-1 which referred to ester sulfate of 930 cm-1 for 3,6 anhydrogalactose and 848 cm-1 assigned to galactose-4-sulfate. From the present investigation, it was observed that sulfate removal and quality improvement with increasing concentration of KOH for cooking seaweed is not statistically significant; therefore, seaweed can be subjected to lower concentration of KOH treatment (6 to 12%) to produce semi-refined carrageenan on commercial scale.

 

Key words:  Seaweed, Kappaphycus alvarezii, KOH treatment, semi-refined carrageenan (SRC), sulfate content, FTIR spectra, gel strength.

Abbreviation

SRC, semi-refined carrageenan; 3,6-AG, 3,6-anhydrogalacotse; FTIR, Fourier Transform Infrared Spectroscopy.