African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12370

Full Length Research Paper

Molecular characterization of bacteria strians isolated from “JIKO”: A herbal preparation consumed in some parts of Kaduna metropolis, Nigeria

Ebipade Kereakede
  • Ebipade Kereakede
  • Department of Biotechnology, Nigerian Defence Academy, Kaduna, Nigeria.
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Mohammed Sani Abdulsalami
  • Mohammed Sani Abdulsalami
  • Department of Biotechnology, Nigerian Defence Academy, Kaduna, Nigeria.
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Nkechi Eucharia Egbe
  • Nkechi Eucharia Egbe
  • Department of Biotechnology, Nigerian Defence Academy, Kaduna, Nigeria.
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Victoria Moltong Yilwa
  • Victoria Moltong Yilwa
  • Department of Biology, Nigerian Defence Academy, Kaduna, Nigeria.
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Enimie Endurance Oaikhena
  • Enimie Endurance Oaikhena
  • Department of Biotechnology, Nigerian Defence Academy, Kaduna, Nigeria.
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Elizabeth Omolola Oladapo
  • Elizabeth Omolola Oladapo
  • Department of Biotechnology, Nigerian Defence Academy, Kaduna, Nigeria.
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Asabe Danjuma
  • Asabe Danjuma
  • Department of Biotechnology, Nigerian Defence Academy, Kaduna, Nigeria.
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Kingsley Onuh
  • Kingsley Onuh
  • Department of Biotechnology, Nigerian Defence Academy, Kaduna, Nigeria.
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  •  Received: 12 October 2020
  •  Accepted: 12 January 2021
  •  Published: 30 April 2021

Abstract

Herbal products are considered as phytomedicines which are used as complementary medicine and without adequate monitoring they are leased straightaway into the market. Preparation, transportation and handling of these products can lead to high microbial contamination which can be harmful to human health. The aim of this research was to molecularly characterize some multiple resistance bacteria obtained from JIKO which is a herbal preparation consumed in some parts of Kaduna metropolis. The genomic DNA was extracted from each bacteria isolates, 16s rRNA genes were amplified and sequenced. The sequenced obtained from the amplified genes were analyzed using Basic Local Alignment Search Tool (BLAST) of the National Center for Biotechnology Institute database to compare the genes with the exist genes with ones in GENbank. The results obtained confirmed the identities of four bacteria isolates: Three Bacillus cereus and a Serratia species.  It was concluded that herbal products can contain some bacterial contaminants if not properly handled.

 

Key words: Herbal, phytomedicines, contaminants, genomic.