African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12405

Full Length Research Paper

Antioxidant properties of polysaccharides from Laetiporus sulphureus in submerged cultures

  Ming-Yeou Lung* and Wei-Zhong Huang        
  Department of Chemical and Materials Engineering, Minghsin University of Science and Technology, Xinfeng, Hsinchu, Taiwan 30401, Republic of China.
Email: [email protected]

  •  Accepted: 26 January 2012
  •  Published: 20 March 2012



Intracellular polysaccharides (IPS) and exopolysaccharides (EPS) are isolated respectively from mycelia and filtrates of submerged culture by Laetiporus sulphureusin a 20-L stirred tank bioreactor with corresponding antioxidant properties investigated. Effective productions of IPS and EPS by submerged cultures of L. sulphureus are available. Number of average molecular weights (Mn) and protein/polysaccharide ratios are 1.29×106 Da and 8.24% for IPS and 3.95×106 Da and 2.38% for EPS. IPS shows an excellent antioxidant property in reducing power and scavenging effect on superoxide anion, whereas EPS has a powerful antioxidant activity in reducing power, chelating effect on ferrous ions and scavenging effect on superoxide anion, as evidenced by their quite low EC50 values below 5 mg/ml. Confirmed by the result is the potential antioxidant application of fermented L. sulphureus polysaccharides in health-promoting functional food and pharmaceutical industries.


Key words: Laetiporus sulphureus, intracellular polysaccharide, exopolysaccharide, antioxidant activity.