African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12269

Full Length Research Paper

Detection of novel polymorphisms in the mitochondrial DNA D-Loop hypervariable region HVI from 400 healthy unrelated individuals from central and North-central Iraq

Ameera, Omran Hussein
  • Ameera, Omran Hussein
  • Department of Molecular Biology, Babylon University, Hilla City, Iraq.
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Imad, Hadi Hameed*
  • Imad, Hadi Hameed*
  • Department of Molecular Biology, Babylon University, Hilla City, Iraq.
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Muhanned, Abdulhasan Kareem
  • Muhanned, Abdulhasan Kareem
  • Babylon University, Centre of Environmental Research, Iraq.
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  •  Received: 30 January 2015
  •  Accepted: 25 March 2015
  •  Published: 08 April 2015

Abstract

The mitochondrial DNA (mtDNA) is a small circular genome located within the mitochondria in the cytoplasm of the cell and a smaller 1.2 kilobase pair fragment, called the control region (D-loop). The aims of this research were to study this region by using the Sanger sequencing technique and establish the degree of variation characteristic of a fragment. FTA® Technology (FTA™ paper DNA extraction) was utilized to extract DNA. PCR products were purified by EZ-10 spin column then sequenced and detected by using the ABI 3730xL DNA Analyzer. Novel polymorphisms discovered at positions 16037, 16075, 16104 and 16201 in future may be suitable sources for identification purpose.

 

Key words:  D-loop, frequency, north-central Iraq, mitochondrial DNA, polymorphism.