African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12083

Full Length Research Paper

Comparative survey of three processes used for the extraction of total phenol content and total flavonoid content of Anacardium occidentale L. and the assessment of its antioxidant activity

MADJITOLOUM BETOLOUM Salomon
  • MADJITOLOUM BETOLOUM Salomon
  • Department of Chemistry. Faculty of Sciences and Techniques University Adam Barka of Abeche (UNABA), Chad.
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TALLA Emmanuel
  • TALLA Emmanuel
  • Department of Chemistry. Faculty of Sciences. University of Ngaoundere (UN), Cameroun.
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NYEMB Jean Noël
  • NYEMB Jean Noël
  • Department of Chemistry. Faculty of Sciences. University of Ngaoundere (UN), Cameroun.
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NGASSOUM Martin Benoît
  • NGASSOUM Martin Benoît
  • Department of Applied Chemistry. National Advanced School Of Agro-Industrial Sciences, University of Ngaoundere (UN) Cameroon.
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TSATSOP TSAGUE Roli Karole
  • TSATSOP TSAGUE Roli Karole
  • Department of Applied Chemistry. National Advanced School Of Agro-Industrial Sciences, University of Ngaoundere (UN) Cameroon.
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MAHMOUT Yaya
  • MAHMOUT Yaya
  • Laboratory of Research on the Natural Substances, University of N’Djamena (UNDJ), Chad.
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  •  Received: 23 October 2017
  •  Accepted: 26 April 2018
  •  Published: 03 October 2018

Abstract

Three processes were used for the extraction of total phenol and total flavonoid contents of Anacardium occidentale L. and to determine its antioxidant activity. Whatever the extraction process was, the total flavonoid and total phenol contents were of the same order. With the microwave-assisted extraction (MAE), 666.07 mg gallic acid equivalent (GAE)/100 g dry matter (DM) of total phenol content was obtained from the stem barks, 601.71 mg/100 g DM from the leaves and 69.18 mg quercetin equivalent (QE)/100 g DM from the nuts of cashew. With Soxhlet extraction, 605.47 mg GAE/100 g DM of total phenol content was obtained from the stem barks, 600.38 mg GAE/100 g DM from the leaves, 609.13 mg GAE/100 g DM from the nuts of cashew, then 67.13 mg QE/100 g DM of the total flavonoid content was obtained from the stem barks, 65.13 mg QE/100 g DM from the leaves and 61.75 mg QE/100 g DM from the nuts of cashew. Finally, with mechanical agitation, 608.52 mg GAE/100 g DM of total phenol content was obtained from the stem barks, 604.85 mg GAE/100 g DM from the leaves and 593.47 mg GAE/100 g DM from the nuts of cashew; 76.86 mg QE/100 g DM of total flavonoid content was obtained from the stem barks, 76.31 mg QE/100 g DM from the leaves and 70.39 mg QE/100 g DM from the nuts of cashew. The antioxidant tests revealed that MAE gave better anti-oxidant activity and more phenolic compounds.

 

Key words: Processes of extraction, antioxidant activity, Anacardium occidentale, microwave-assisted extraction.