African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12500

Full Length Research Paper

Molecular cloning and characterization of an actin-depolymerizing factor gene in Hevea brasiliensis

Zhi Deng1,2, Xianghong Liu1,3,4, Chunliu Chen1,2, Weimin Tian1,2, Zhihui Xia3* and Dejun Li1,2*
  1Key Laboratory of Rubber Biology, Ministry of Agriculture, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, Hainan 571737, China. 2Hainan Provincial Key Laboratory of Tropical Crops Cultivation and Physiology, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, Hainan, 571737, China. 3Institute of Biological Science and Technology, College of Agriculture, Hainan University, Haikou, 570228, China. 4State Key Laboratory of Tropical Crop Biotechnology, Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China.
Email: [email protected], [email protected].

  •  Accepted: 23 September 2010
  •  Published: 08 November 2010

Abstract

 

Actin-depolymerizing factor (ADF) plays important roles in regulating actin dynamics by maintaining the optimum equilibrium between unpolymerized actin molecules and assembled actin filaments in different cellular processes. In this study, the first ADF gene in Hevea brasiliensis designated as HbADF, was isolated. The HbADFcontained an open reading frame (ORF) encoding 139 amino acids. The deducedHbADF showed high identities to plants ADF proteins. Besides a conserved ADF domain, HbADF also contained putative actin and specific F-actin binding sites, phosphorylation site and possible CAM (calmodulin) combining region. The phylogenetic analysis indicated that HbADF was clustered in the subclass I. Being consistent with phylogenetic result, the expression of HbADF was constitutive. TheHbADF transcripts were upregulated by ethephon and wounding treatments; whereas, HbADF was firstly induced, and then gradually downregulated by jasmonic acid. The expression profiles and characterizations of HbADF suggested that HbADF might be associated with latex regeneration and flow in H. brasiliensis.

 

Key words: Actin cytoskeleton, actin-depolymerizing factor, expression analysis,Hevea brasiliensis, semi-quantitative reverse-transcription polymerase chain reaction.

Abbreviation

Abbreviations: ADF, Actin-depolymerizing factor; ABPs, actin binding proteins; bp, base pair; cDNA, complementary DNA; ET, ethephon; JA, jasmonic acid; EST,expression sequence tag; F, forward primers; R, reverse primers; NCBI, National Center for Biotechnology Information; ORF, open reading frame; RACE, rapid amplification of complementary DNA ends; CAM, calmodulin; RT-PCR, reverse-transcription polymerase chain reaction.

Copyright © 2025 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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