African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12196

Full Length Research Paper

Genetic diversity and historical demography of kuruma shrimp (Penaeus japonicus) species complex off China based on mitochondrial DNA analysis

Chun-Han Shih1, Hsien-Lu Haung2, Ta-Jen Chu3, Ying-Chou Lee1, Ching-Ming Wang4 and Tzong-Der Tzeng5*
  1Institute of Fisheries Science, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 106, Taiwan. 2Department of Nutrition and Health Science, Fooyin University, 151, Chinhsueh Road, Ta-liao, Kaohsiung. 3Department of Leisure and Recreation Management, Chung Hua University, 707 sec. 2, Wu Fu Road, Hsin Chu 300, Taiwan. 4Graduate Institute of Environmental Education National Taiwan Normal University, Taipei 116, Taiwan. 5Department of Leisure, Recreation and Tourism Management, Shu-Te University, Kaohsiung County 824, Taiwan.
Email: [email protected]

  •  Accepted: 03 January 2011
  •  Published: 14 February 2011

Abstract

 

Two varieties (I and II) of kuruma shrimp (Penaeus japonicus) were found in the north of South China Sea (SCS) and Taiwan Strait (TS). To estimate the demographic history and genetic diversity of this species complex off China, 141 individuals were collected from the East China Sea (ECS), TS and SCS and 27 variety 2 specimens from SCS were also sampled for comparison. Sequence analyses on fragments of 454-bp at 5’ end of mitochondrial DNA control region were conducted. Neighbor-joining tree and network of all populations yielded two clades; one included variety I individuals, the other comprised variety II. The variety II could also be found in ECS. The haplotype diversity (h) for variety I was high for all populations (99.9%), with values from 99.3% (ECS) to 1 (SCS). Nucleotide diversity (π) for variety I was low for all populations (0.0321), with values from 0.0285 (TS) to 0.0361 (ECS). The h and π for variety II were 1 and 0.0446, respectively. Analyses of molecular variance and FST revealed no significant genetic structure for variety I populations. Neutrality tests and mismatch distribution analyses suggested a late Pleistocene population expansion for both variety I (62,132 to 86,605 years ago) and variety II (94,464 to 146,655 years ago) of kuruma shrimp off China.

 

Key words: Mitochondrial DNA, Penaeus japonicus, historical demography, control region.

Abbreviation

SCS, South China sea; TS, Taiwan strait; ECS, East China sea;mtDNA, mitochondiral DNA; PCR, polymerase chain reaction; AMOVA, Analysis of molecular variance.

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