African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12500

Full Length Research Paper

Shear stress and interleukin-8 (IL-8) on the proliferation, differentiation and tube formation of endothelial progenitor cells (EPCs)

Zhou Shu1,2,3#, Yi Lai4#, Ting-He Yu1,2,3, Ling-Juan Ling Hu1,2,3 and Yi Zhang1,2,3*
1Chengdu1Department of Obstetric and Gynecologic, West China Second University Hospital, Sichuan University, Chengdu, 610041, China. 2Laboratory of Biomedical Ultrasonics and Gynecologic cancers, West China Second University Hospital, Si Chuan University, Chengdu 610041, China. 3Key Laboratory of Obstetric and Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, Sichuan, China. 4Chengdu Women and Children’s Medical Center Hospital, Chongqing Medical University, Chongqing, China.   #Both authors contributed equally to this work.
Email: [email protected]

  •  Accepted: 24 October 2011
  •  Published: 21 December 2011

Abstract

Endothelial progenitor cells (EPCs) derived from bone marrow, are also found in circulation and involved in both tumor vasculogenesis and wound healing. During the process of EPCs incorporation into tissues and neovascularization, the cells are exposed to fluid shear stress. Interleukin-8 (IL-8) has been shown to play an important role in tumor growth, angiogenesis and metastasis. In our previous study, IL-8 gene expression of cultured human umbilical vein endothelial cells was significantly increased after stimulation by low shear stress for 1 to 2 h. Hence, there may be some connection between the IL-8 and shear stress in the EPCs incorporation into tissues and neovascularization. This study aimed to investigate whether the shear stress and IL-8 played important role in this process. After the cells were pretreated by shear stress, the cells were stimulated by IL-8, and then the expressions of CD133, VWF, VE-cadherins and VEGFR-2 in EPCs were measured. The proliferation, apoptosis, cell cycle, migration and tube formation of EPCs were detected after the cells expose to shear stress stimulated by IL-8. Results show that IL-8 or shear stress alone can induce EPCs proliferation decreased. 5 and 10 dyne/cm2 shear stress can both induce EPCs differentiation into the mature ECs. The shear stress of 10 dyne/cm2 on the EPCs developed an extensive tubular network than that of cells stimulated by IL-8 alone, but the shear stress of 5 dyne/cm2 depressed the tube formation ability. From the result of transwell assay, shear stress of 10 dyne/cm2 enhanced the EPCs cell migration stimulated by IL-8, while shear stress of 5 dyne/cm2 decreased it. In addition, the shear stress of 10 dyne/cm2 induced an increase in wound healing. These findings suggest that EPCs are sensitive to shear stress. Both shear stress and IL-8 can influence the process of EPCs repair in wound. Also, high shear stress (10 dyne/cm2) combined with IL-8 can induce the differentiation of EPCs to more matured endothelial cells.

 

Key words: Shear stress, interleukin-8 (IL-8), endothelial progenitor cells (EPCs).

Abbreviation

EPCs, Endothelial progenitor cells; IL-8, interleukin-8.

Copyright © 2025 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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