African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12179

Full Length Research Paper

Production and characterization of proteases from edible mushrooms cultivated on amazonic tubers

Ana Rita Gaia Machado
  • Ana Rita Gaia Machado
  • Universidade Federal do Amazonas, Rede de Biodiversidade e Biotecnologia da Amazônia Legal –Bionorte, Av. General Rodrigo Octavio Jordão Ramos, 1200 - Coroado I, Manaus - AM, 69067-005, Brazil.
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Salomao Rocha Martim
  • Salomao Rocha Martim
  • Universidade Federal do Amazonas, Rede de Biodiversidade e Biotecnologia da Amazônia Legal –Bionorte, Av. General Rodrigo Octavio Jordão Ramos, 1200 - Coroado I, Manaus - AM, 69067-005, Brazil.
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Mircella Marialva Alecrim
  • Mircella Marialva Alecrim
  • Departamento de Engenharia Agrícola e de Solos–UFAM. Faculdade de Ciências Agrárias, Universidade Federal do Amazonas, Av. General Rodrigo Octávio, 6200, Coroado I, Cep: 69077-000, Manaus, Amazonas, Brazil.
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Maria Francisca Simas Teixeira
  • Maria Francisca Simas Teixeira
  • Departamento de Parasitologia, Universidade Federal do Amazonas, Instituto de Ciências Biológicas, Coleção de Culturas DPUA –UFAM. Av. General Rodrigo Octávio, 6200, Coroado I, Cep: 69077-000, Manaus, Amazonas, Brazil.
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  •  Received: 15 July 2017
  •  Accepted: 11 October 2017
  •  Published: 15 November 2017

Abstract

Proteases are important commercial enzymes, and among their numerous sources are the Basidiomycetes. The use of proteases in many industrial areas promotes the search for enzymes with new properties. The aim of this study is to produce and characterize peptidases of a biocomposite from mycelial biomass grown in Amazonic tubers. Lentinus citrinus DPUA 1535 and Pleurotus ostreatoroseus DPUA 1720 were cultivated on Dioscorea trifida, Manihot esculenta and Dioscorea alata supplemented with rice bran or manioc flour residue in different proportions. The highest proteolytic activity was determined in the crude extract from P. ostreatoroseus grown in D. alata (DA) without supplementation (142.22 U/mL). The enzymes showed optimum activities at 40°C and pH 7.0; and stability at 50°C and pH 8.0. The proteases were classified as cysteine proteases based on the effect of inhibitors used.

 

Key words: Pleurotus, Lentinus, Dioscorea, protease.