African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12392

Full Length Research Paper

Antioxidant activity of essential oil of three cultivars of Amomum subulatum and standardization of high performance thin layer chromatography (HPTLC) method for the estimation of 1,8-cineole

Aftab Alam
  • Aftab Alam
  • Department of Biotechnology, School of Engineering and Technology, Sharda University, Greater Noida, India.
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Rita Singh Majumdar
  • Rita Singh Majumdar
  • Department of Biotechnology, School of Engineering and Technology, Sharda University, Greater Noida, India.
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  •  Received: 03 May 2018
  •  Accepted: 28 June 2018
  •  Published: 05 September 2018

Abstract

Essential oils of the fruits of three cultivars of Amomum subulatum (Family-Zingiberaceae), such as varlangy, seremna and sawney were isolated. Antioxidant potential was determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) scavenging and FeCl3-reducing models and the presence of 1,8-Cineole was quantified using the developed high performance thin layer chromatography (HPTLC) method. In the DPPH scavenging model, the IC50 value of the essential oil of the seremna, varlangy and sawney were found to be 172.3, 216.9 and 274.3 μg/ml, respectively, while in the ABTS scavenging model, the IC50 value of the seremna, varlangy and sawney were found to be 27.96, 31.34 and 32.49 μg/ml respectively. The antioxidant power of the FeCl3 reducing model, the absorbance value of the essential oil of the seremna was found comparatively higher than the essential oils of varlangy and sawney. The content of 1,8-cineole was determined by the developed HPTLC densitometric method, and the value of the percentage (mean ± SD, % w/w) content of the essential oils  of the seremna, varlangy and sawney were found to be 69.59 ± 1.45%, 48.78 ± 3.21 and  47.84 ± 1.76 respectively. The highest antioxidant value of seremna cultivar may be due to the presence of high content of 1, 8-cineole. The HPTLC method, therefore confirms that monoterpene 1,8-cineole is the main antioxidant compound present in the fruits of A. subulatum.

 

Key words: Amomum subulatum, cultivars, essential oil, 1,8-cineole, high performance thin layer chromatography (HPTLC), antioxidants.