African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12258

Full Length Research Paper

Cholic acid resistance and the adherence ability of Bifidobacterium pseudocaenulatum G4

Ali Qahtan Sulaiman1, Farid Azizi Jalilian2, Shuhaimi Mustafa3, Zamberi Sekawi2, Hasanah Mohd Ghazali1, Anis Shobirin Meor Hussin1 and Abd Manap Yazid1*
  1Faculty of Food Science and Technology, University Putra Malaysia, 43400, Selangor, Malaysia. 2Faculty of Medicine and Community Health, University Putra Malaysia, 43400, Selangor, Malaysia. 3Halal Products Research Institute, University Putra Malaysia, 43400, Selangor, Malaysia.  
Email: [email protected]

  •  Accepted: 29 April 2009
  •  Published: 05 October 2009

Abstract

 

The adherence capacity of Bifidobacterium longum BB536 and Bifidobacterium pseudocatenulatum G4 on HT-29 human epithelium cell line with the presence of cholic acid were assessed. B. longum BB536 showed a higher adhesion level on HT-29 human epithelium cell line compared to B. psudocatenulatum G4. However, in the presence of physiological concentration (0.094 and 0.94 µM) of cholic acid, the adhesion level of Bifidobacterium strains dropped between 5 and 55% respectively, depending on pH, time and strain. The adaptation of Bifidobacteriumstrains to cholic acid was shown to be increased with time. It was concluded that the acquisition of cholic acid resistance by those Bifidobacterium strains promoted changes in the adhesion ability on HT-29 human epithelium cell line.

 

Key words: Adhesion, bifidobacterium, cholic acid.