African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12258

Full Length Research Paper

Growth regulators, DNA content and anatomy in vitro-cultivated Curcuma longa seedlings

Dirlane Antoniazzi
  • Dirlane Antoniazzi
  • Pós-graduação em Biotecnologia Aplicada à Agricultura, University Paranaense, Pç. Mascarenhas de Moraes, 4282, CEP 87502-210, Umuarama, Brazil.
  • Google Scholar
Meire Pereira de Souza Ferrari
  • Meire Pereira de Souza Ferrari
  • Pós-graduação em Biotecnologia Aplicada à Agricultura, University Paranaense, Pç. Mascarenhas de Moraes, 4282, CEP 87502-210, Umuarama, Brazil.
  • Google Scholar
Andressa Bezerra Nascimento
  • Andressa Bezerra Nascimento
  • Pós-graduação em Biotecnologia Aplicada à Agricultura, University Paranaense, Pç. Mascarenhas de Moraes, 4282, CEP 87502-210, Umuarama, Brazil.
  • Google Scholar
Flavia Aparecida Silveira
  • Flavia Aparecida Silveira
  • Departamento de Fitotecnia, University Federal de Lavras, Caixa Postal 3037, CEP 37200-000, Lavras, Brazil.
  • Google Scholar
Leila Aparecida Salles Pio
  • Leila Aparecida Salles Pio
  • Departamento de Fitotecnia, University Federal de Lavras, Caixa Postal 3037, CEP 37200-000, Lavras, Brazil.
  • Google Scholar
Moacir Pasqual
  • Moacir Pasqual
  • Departamento de Fitotecnia, University Federal de Lavras, Caixa Postal 3037, CEP 37200-000, Lavras, Brazil.
  • Google Scholar
Helida Mara Magalhaes
  • Helida Mara Magalhaes
  • Departamento de Fitotecnia, University Federal de Lavras, Caixa Postal 3037, CEP 37200-000, Lavras, Brazil.
  • Google Scholar


  •  Received: 01 May 2016
  •  Accepted: 25 July 2016
  •  Published: 10 August 2016

Abstract

Curcuma longa L., from the Zingiberaceae family, generally reproduces through its rhizomes, which are also utilized for therapeutic purposes because they are rich in terpenoids. Its conventional propagation has low efficiency due to the small number of seedlings and their contamination by pathogens. Therefore, this study aimed to evaluate the influence of growth regulators on the development of in vitro-cultivated C. longa as well as to determine their influence on DNA content and foliar anatomy. Shoots were inoculated in MS culture medium with the addition of 30 g/L of sucrose and 6.5 g/L of agar, and a pH adjusted to 5.8. Two assays were built to study the multiplication and rooting phases of growth. The first assay evaluated the influence of eight concentrations of cytokinins and auxins on the multiplication phase. Leaf samples were analyzed for DNA content through flow cytometry, utilizing two reference standards, green pea, and tomato. Characteristics of leaf anatomy were also measured in four time periods. The second assay analyzed the influence of six auxin concentrations on the rooting phase. The first assay showed that the root systems grew more in treatment 3 (4.44 µM benzylaminopurine [BAP], 0.46 µM kinetin [KIN]) and reached greater dry mass in T8 (8.88 µM BAP, 0.92 µM KIN, 2.16 µM naphthalene acetic acid [NAA]). The largest fresh matter of the main shoot was found in T2 (4.44 µM BAP). The estimated DNA content varied depending on the presence of supplemental growth regulators, from 2.38 to 2.77 pg, and was greater in T4 (4.44 µM BAP, 1.08 µM NAA) and T5 (4.44 µM BAP, 0.46 µM KIN, 1.08 µM NAA). Results from the latter two treatments were not significantly different. Estimates of DNA content were precise, as indicated by coefficients of variation that were much lower than 5%. The results also showed quantitative variation of evaluated anatomical traits. In general, there was a thin epidermis layer with rectangular cells, followed by parenchyma with octahedral cells and differentiated xylem and phloem. In leaf parenchyma, the presence of idioblasts containing phenolic compounds was observed in all growth stages. In the rooting phase, the supplementary auxins affected the dry matter of the aerial part and roots. The highest averages were observed in treatments with 2.0 µM NAA.

 

Key words: Turmeric, micropropagation, flow cytometry, vegetal anatomy.